Cytotoxicity profiling of choline chloride-based natural deep eutectic solvents
Creators
- 1. Chemistry and Biochemistry Laboratory, Department of Field and Vegetable Crops, Faculty of Agriculture, University of Novi Sad Trg Dositeja Obradovića 8 21000 Novi Sad Serbia boris.popovic@polj.uns.ac.rs +381 21 450 857 +381 21 485 3424.
- 2. Department of Experimental Oncology, Institute for Oncology and Radiology of Serbia Pasterova 14 11000 Belgrade Serbia.
- 3. iBET, Instituto de Biologia Experimental e Tecnológica Avenida da República, Quinta do Marquês, 2780-157 Oeiras Portugal. ; Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA) Avenida da República, Quinta do Marquês, 2780-157 Oeiras Portugal.
Description
Abstract. This study aims to examine in detail for the first time the cytotoxic profile of twelve choline chloride-based deep eutectic solvents (NADES) against HT-29, Caco-2, MCF-7, and MRC-5 cell lines. All NADES systems were synthesized by microwave synthesis using choline chloride as a hydrogen bond acceptor (HBA) and selected sugars, alcohols, organic acids, and urea as hydrogen bond donors (HBD) with the addition of 20% water (w/w) to all systems. It was observed that the cytotoxic effect predominantly depended on the structure of HBD. Acidic systems, where HBDs were organic acids showed the highest cytotoxic effects in all investigated cell lines. The cytotoxicity depended mostly on the concentration of the NADES system in the cell medium as well as on the chemical constitution of the investigated systems. The highest cytotoxic effects showed acidic systems, especially to the HT-29 cell line. The EC50 value for the citric acid-based system was 3.91 mg mL−1 for the HT-29 cell line which was the most vulnerable to acidic NADES systems.
Notes
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Cytotoxicity profiling of choline chloride-based.pdf
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Additional details
Related works
- Is derived from
- rsc.li/rsc-advances (Handle)
- Is documented by
- 36756554 (PMID)
- Is identical to
- PMC9891074 (pmcid)
- Is part of
- 2046-2069 (ISSN)
- Is supplemented by
- 10.1039/d2ra07488e (DOI)
Funding
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200043 (Institute of Oncology and Radiology of Serbia, Belgrade) 200043
- Ministry of Education, Science and Technological Development
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200117 (University of Novi Sad, Faculty of Agriculture) 200117
- Ministry of Education, Science and Technological Development