CAPILLARY ELECTROPHORESIS

Capillary electrophoresis [CE] is an analytical technique performed in a thin diameter glass tube that separate ions based on their electrophoretic mobility with help of an applied voltage. CE plays an ideal role in the analysis of highly polar charged analytes, analysis of both basic, clinical pharmaceutical, in the analysis and characterization of macromolecules like proteins and carbohydrates. It is often used when rapid results are desired. In this technique, separation is based on the size and charge of the molecules or ions. Capillary electrophoresis is most predominately used because it gives faster results and provide high resolution separation. The rate at which the particle moves is directly proportional to the applied electric field that is, the greater the field strength, the faster the mobility. It is a useful technique because there is a large range of detection methods available.