Impact of animal saliva on the performance of rapid antigen tests for detection of SARS-CoV-2 (wildtype and variants B.1.1.7 and B.1.351)
- 1. Friedrich-Loeffler-Institut, Institute of Novel and Emerging Infectious Diseases, 17493 Greifswald - Insel Riems, Germany
Description
SARS-CoV-2 infects several animal species and SARS-CoV-2 variants of concern (VOCs) may even show (as in
humans) enhanced inter- and intra-species transmission rates. We correlated sensitivity data of SARS-CoV-2
rapid antigen tests (RATs) to viral RNA genome equivalents analyzed by real-time reverse transcriptasepolymerase
chain reaction (RT-PCR). Further, we checked their suitability for testing animals by assessing
saliva and VOC effects. Viral loads up to 2 logs (RNA copy number) under the hypothetical SARS-CoV-2 infectivity
threshold were detected by most analyzed RATs. However, while saliva from various animal species
showed generally no adverse effects on the RATs’ analytical sensitivities, the detection of VOCs B.1.1.7 and
B.1.351 was in some RATs inferior to non-VOC viruses.
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- Is published in
- Journal article: PMC8452372 (pmcid)
- Journal article: 10.1016/j.vetmic.2021.109243 (DOI)