Published December 23, 2022 | Version v1
Journal article Open

Molecular diagnostics for monitoring insecticide resistance in the western flower thrips Frankliniella occidentalis

Creators

  • 1. Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, GR-70013 Heraklion, Greece
  • 2. Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, GR-70013 Heraklion, Greece & Institute of Olive Tree, Subtropical Crops and Viticulture, Hellenic Agricultural Organization "DIMITRA", 70013 Heraklion, Greece.
  • 3. Institute of Olive Tree, Subtropical Crops and Viticulture, Hellenic Agricultural Organization "DIMITRA", 70013 Heraklion, Greece
  • 4. Laboratory of Translational Oncology, School of Medicine, University of Crete, Heraklion, Greece
  • 5. Institute of Olive Tree, Subtropical Crops and Viticulture, Hellenic Agricultural Organization "DIMITRA", 70013 Heraklion, Greece.
  • 6. Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, GR-70013 Heraklion, Greece & Pesticide Science Laboratory, Department of Crop Science, Agricultural University of Athens, GR-11855 Athens, Greece.

Description

BACKGROUND: Insecticide resistance has emerged in various western flower thrips (WFT) populations across the world, threatening the efficiency of chemical control applications. Elucidation of insecticide resistance mechanisms at the molecular level provides markers for the development of diagnostics, to monitor the trait and support evidence-based resistance management.

RESULTS: TaqMan and Droplet Digital PCR (ddPCR) diagnostics were developed and validated, against Sanger sequencing, in individual and pooled WFT samples, respectively, for the: G275E mutation (nicotinic acetylcholine receptor α6 gene- nAChR α6) associated with resistance to nAChR allosteric modulators - site I (spinosyns), L1014F, T929I, T929C and T292V mutations (voltage-gated sodium channel gene- vgsc) linked with pyrethroid resistance and I1017M (chitin synthase 1 gene- chs1) conferring resistance to growth inhibitors affecting CHS1 (benzoylureas). The detection limits of ddPCR assays for mutant allelic frequencies (MAFs) were in the range of 0.1%-0.2%. The assays were applied in nine WFT field populations from Crete, Greece. The G275E (MAF = 29.66%-100.0%), T929I and T929V (combined MAFs = 100%), L1014F (MAF = 11.01%-37.29%) and I1017M (MAF = 17.74%-51.07%) mutations, were present in all populations.

CONCLUSION: The molecular diagnostics panel that was developed in this study can facilitate the quick and sensitive pesticide resistance monitoring of WFT populations at the molecular level, to support evidence-based IRM strategies.

Notes

This is the peer reviewed version of the following article: "Mavridis K, Ilias A, Papapostolou KM, Varikou K, Michaelidou K, Tsagkarakou A, Vontas J. Molecular diagnostics for monitoring insecticide resistance in the western flower thrips Frankliniella occidentalis. Pest Manag Sci. 2022 Dec 23", which has been published in final form at https://doi.org/10.1002/ps.7336. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley's version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.

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Additional details

Funding

SuperPests – Innovative tools for rational control of the most difficult-to-manage pests (super pests) and the diseases they transmit 773902
European Commission