Published February 6, 2023 | Version v1
Journal article Open

Gingival orofacial granulomatosis clinical and 2D/3D microscopy features after orthodontic therapy: a pediatric case report

Description

Supplementary Figure S1: Polarized acquisition of red picrosirius staining from healthy (A), plaque-induced inflamed gingiva (B) and OFG gingival sample. Note the strong ECM fibers disappearance in OFG gingival sample compared to healthy gingiva.

Supplementary Figure S2: Mast cells localization (white) by anti-tryptase immunofluorescence highlighted similar distribution between healthy (A), plaque-induced inflamed gingiva (B) and OFG (C) gingival samples. Cell nuclei stained by DAPI.

Supplementary Figure S3: Healthy gingiva 3D microscopy emphasized few leucocytes (white arrows) in deep connective tissue and no granulomas.

Supplementary Movie S1 : 3D OFG gingival granulomas movie (12x).

Supplementary Movie S2 : Whole LSFM stack acquisition movie of gingival OFG sample (5x).

Supplementary Movie S3: LSFM OFG gingival focus stack acquisition movie (12x).

Supplementary Movie S4: LSFM OFG gingival granuloma focus stack acquisition movie.

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