Accurate Diagnosis of COVID-19 from Self-Collectable Biospecimens Using Synthetic Apolipoprotein H Peptide-Coated Nanoparticle Assay
Creators
- 1. Copernicus Integrated Solutions for Biosafety Risks (CISBR), Mauguio 34130, France; ApoHTechnologies, La Grande Motte 34280, France; UMR5151/ French Research Institute for Development (IRD), University of Montpellier (UM), Montpellier 34093, France
- 2. Department of Internal Medicine, Seoul National University College of Medicine, Seoul 03080, Korea
- 3. Interdisciplinary Program in Bioengineering, Seoul National University, Seoul 08826, Korea; Bio-MAX Institute and Department of Electrical and Computer Engineering, Seoul National University, Seoul 08826, Korea; QuantaMatrix Inc., Seoul 08506, Korea; Biomedical Research Institute, Seoul National University Hospital, Seoul 03080, Korea
- 4. Interdisciplinary Program in Bioengineering, Seoul National University, Seoul 08826, Korea; Integrated Major in Innovative Medical Science, Seoul National University, Seoul 03080, Korea
- 5. Interdisciplinary Program in Bioengineering, Seoul National University, Seoul 08826, Korea
- 6. Bio-MAX Institute and Department of Electrical and Computer Engineering, Seoul National University, Seoul 08826, Korea
- 7. QuantaMatrix Inc., Seoul 08506, Korea; Interdisciplinary Program in Cancer Biology, Seoul National University College of Medicine, Seoul 03080, Korea
- 8. QuantaMatrix Inc., Seoul 08506, Korea
- 9. Department of Electrical and Computer Engineering, Seoul National University, Seoul 08826, Korea
- 10. International Vaccine Institute, Seoul 08826, Korea
Description
A high-throughput, accurate screening is crucial for
the prevention and control of severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2). Current methods, which involve
sampling from the nasopharyngeal (NP) area by medical staffs,
constitute a fundamental bottleneck in expanding the testing
capacity. To meet the scales required for population-level
surveillance, self-collectable specimens can be used; however, its
low viral load has hindered its clinical adoption. Here, we describe a
magnetic nanoparticle functionalized with synthetic apolipoprotein
H (ApoH) peptides to capture, concentrate, and purify viruses. The
ApoH assay demonstrates a viral enrichment efficiency of >90% for
both SARS-CoV-2 and its variants, leading to an order of magnitude
improvement in analytical sensitivity. For validation, we apply the
assay to a total of 84 clinical specimens including nasal, oral, and mouth gargles obtained from COVID-19 patients. As a result, a
100% positivity rate is achieved from the patient-collected nasal and gargle samples, which exceeds that of the traditional NP swab
method. The simple 12 min pre-enrichment assay enabling the use of self-collectable samples will be a practical solution to overcome
the overwhelming diagnostic capacity. Furthermore, the methodology can easily be built on various clinical protocols, allowing its
broad applicability to various disease diagnoses.
Files
ApoH ultrasensitive detection SARSCoV.pdf
Files
(4.4 MB)
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