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Published January 26, 2023 | Version 1.0
Preprint Open

Problem of plastic container for storage of chemicals for nucleic acid isolation kits for PCR use

  • 1. Genekam Biotechnology AG

Description

There is a lot of use of plastic products in the biotechnology field. This report of RNA isolation kit is showing that there is a reaction taking place with a chemical (guanidine thiocyanate) in storage bottle during the self-life period as this liquid changed its color from transparent to yellow along with building the precipitation at the bottom. It makes the chemical unsuitable for use. The problem was solved through replacing the storage container from other manufacturer. Therefore, there is a need to select the storage bottles carefully for long term storage of chemicals to be used in biotechnological methods.

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References

  • Bhatia, Sudhir (2022): Pitfalls found in SARS CoV-2 specific test performance during the comparison between WHO recommended method and a commercial test. figshare. Preprint. https://doi.org/10.6084/m9.figshare.19035722.v1
  • -Dennis R. Jenke, Jill M. Jene, Mitchell Poss, James Story, Tom Tsilipetros, Alex Odufu, William Terbush, Accumulation of extractables in buffer solutions from a polyolefin plastic container, International Journal of Pharmaceutics, Volume 297, Issues 1–2, 2005, Pages 120-133, ISSN 0378-5173, https://doi.org/10.1016/j.ijpharm.2005.03.010.
  • McGookin R. RNA extraction by the guanidine thiocyanate procedure. Methods Mol Biol. 1985;2:113-6. doi: 10.1385/0-89603-064-4:113. PMID: 21374179.
  • Bhatia, Sudhir (2021): Loss of an abundant quantity of ribonucleic acid during mini column isolation method. figshare. Preprint. https://doi.org/10.6084/m9.figshare.17108156.v1