In-Vitro ANTIMICROBIAL SYNERGISTIC AND ANTI-TB ACTIVITIES OF PHYLLANTHUS ACIDUS METHANOLIC EXTRACT

The objective of the present study was to evaluate the synergistic
antimicrobial and anti-tubercular activities of phyllanthus acidus
Methanolic extract. Antibacterial study was carried out by plate hole
diffusion or agar well diffusion assay to determine the growth
inhibition of bacteria. Antifungal activity was performed by the use
of Saubouraud dextrose agar medium (SDA). The synergistic activity
study was calculated by means of cup plate method (Kirbauy bauer
technique) using two wells in a plate. The Methanolic plant extract of
Phyllanthus acidus (500μg/ml) was used in combination with
Oxytetracycline (500μg/ml). Anti-tubercular assay was performed
using Micro plate Alamar Blue Assay (MABA) using the suspension
of Mycobacterium tuberculosis H37Rv strain. The concentrations of
plant extract were used are 1000 μg/ml, 500μg/ml, 250μg/ml, 125
μg/ml, 62.5μg/ml. The medicinal plant appear to have a broad
antimicrobial activity spectrum, they could be useful in antiseptic and
disinfectant formulation as well as in anti-tubercular activity. Among
the various micro organisms, the Methanolic extract was more active
against Micrococcus flavum. In antifungal activity the Methanolic
extract shows positive results for all fungus. The anti-tubercular
activity was compared with standard drug Rifampicin. The
Methanolic extract was having more percentage inhibition when
compared to other extracts.