TO DEVELOP A VALIDATED ANALYTICAL METHOD FOR THE SIMULTANEOUS ESTIMATION OF METFORMIN, GLICLAZIDE AND VOGLIBOSE BY RP-HPLC

Another technique was built up for concurrent estimation of Metformin and Gliclazide and Voglibose RP-HPLC strategy. Metformin and Gliclazide was unreservedly solvent in water and liquor. Voglibose was uninhibitedly solvent in liquor and sparingly dissolvable in water. Methanol and potassium dihydrogen ortho phosphate ( pH 3) was picked as the versatile stage. The run season of the HPLC strategy was 5 minutes. The strategy was approved for framework appropriateness, linearity, exactness, precision, explicitness, roughness vigor, LOD and LOQ. The framework appropriateness boundaries were inside breaking point, subsequently it was presumed that the framework was reasonable to play out the examine. The strategy shows linearity between the focus scope of 10-100 µg/ml. The % recuperation of Metformin, Gliclazide and Voglibose were seen as in the scope of 99.23 % - 98.11 %. As there was no impedance due to excipients and portable stage, the technique was seen as explicit. The technique was powerful and rough as seen from immaterial variety in the consequences of examination by changes in Flow rate and Mobile stage sythesis independently and investigation being performed by various investigators.

Keywords: Metformin, Gliclazide and Voglibose, RP‐HPLC, Simultaneous estimation.