Published April 20, 2023 | Version v1
Dataset Open

Mechanism of RecF-RecO-RecR cooperation in bacterial homologous recombination

Creators

  • 1. Laboratory of Protein Structure, International Institute of Molecular and Cell Biology in Warsaw, Warsaw, Poland
  • 2. Biophysics Core Facility, International Institute of Molecular and Cell Biology in Warsaw, Warsaw, Poland

Description

This repository includes the following source datasets:

1. FluorescenceAnisotropyIII.zip

This contains following files:

  1. The raw fluorescence anisotropy file (Excel file) showing the values for Polarization, Anisotropy, Total Intensity, Intensity (parallel) and Intensity (perpendicular) for all the samples included in the paper as separate sheets.
  2. The analysis files generated in the Graphpad prism.
  3. The details of the files.

 

2. FT-IR.zip: This contains the normalized values for the FT-IR spectroscopy of the WT RecF, WT RecR, RecF A170W and RecR A147W.

 

3. Glycerol gradient.zip: This contains the raw gel files for the three sets of independent experiments, standard protein ruler with the respective WT RecF, WT RecO and WT RecR and details regarding the loading of samples on gels.

 

4. Pull-downII.zip: This contains details of all the pull-down experiments and the raw gel files used for

  1. Quantification
  2. The major samples for all the experiments done in triplicate
  3. Figure preparation for paper

 

 

Files

FluorescenceAnisotropyIII.zip

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