Sex-specific changes in autosomal methylation rate in ageing common terns - Code
Contributors
Researchers:
Supervisors:
- 1. Max-Planck-Society
- 2. Institute of Avian Research
Description
In the manuscript "Sex-specific changes in autosomal methylation rate in aging common terns" published in Frontiers Ecology And Evolution, we investigate sex-specific age effects in global DNA methylation patterns in aging common terns in a longitudinal study. We collected blood at 1-, 3- and/or 4-year intervals, extracted DNA from the erythrocytes and estimated autosomal DNA methylation by mapping Reduced Representative Bisulfite Sequencing reads to a de novo assembled reference genome.
The raw RRBS reads can be found at: https://www.ebi.ac.uk/ena, PRJEB48910 and the genome at https://www.ncbi.nlm.nih.gov/, PRJNA560234.
This document combines the various scripts and programs used for the Reduced Representative Bisulfite Sequencing data analyses and GLMMs (e.g. RefFreeDMA, picard, bismark, and R) into one combined script to make the work reproducible. We further share the final data frame used for the GLMM under another DOI: 10.5281/zenodo.7533890.
Notes
Files
Bioinformatic_code.md
Files
(33.7 kB)
Name | Size | Download all |
---|---|---|
md5:92724aac248f2c6dac9b1cbd2c2d2e87
|
33.7 kB | Preview Download |
Additional details
Related works
- Compiles
- Dataset: 10.5281/zenodo.7533890 (DOI)
- Is published in
- Journal article: 10.3389/fevo.2023.982443 (DOI)