PHYTOCHEMICALS & ANTI HYPERLIPIDAEMIC ACTIVITY OF METHANOLIC EXTRACT OF TEPHROSIA PURPUREAON RATS

Natural products, particularly of plant origin, are the main quarry for discovering promising lead candidates and play an imperative role in the upcoming drug development programs. Ease of availability, low cost, and least side effects make plant-based preparations the main key player of all available therapies. Tephrosia purpurea is a wild herb belongs to the family fabaceae and commonly known as sharpunkha. This plant has also been used for the treatment of several gastrointestinal disorders and has ability to cure disorder related to bowel, kidney liver spleen. Its dried parts can be used effectively for the treatment of boils, bleeding piles, bronchitis etc. It also has diuretic property. Its roots decoction is useful in enlargement and damage of liver. The aim of this study is to examine anti-hyperlipidaemic effect of  Tephrosia purpurea. The leaves of plant were collected and subjected to extraction by methanol as solvent. Further qualitative & quantitative estimation of phytochemical and antioxidant activity was also checked. The anti -hyperlipidaemic activity was analysed in rats by examining various biochemical parameters. The results showed that the yields were found to be 7.28% w/w of Tephrosia purpurea of petroleum ether extract, 12.54 % w/w of methanolic extract. The phytochemical screening revealed presence of alkaloid, flavonoid glycosides, tannins, steroid, phenol. Total phenol & flavonoid content was found to be 0.865 mg/100mg and 0.947 mg/100mg of dried extract respectively. The IC 50 value for Methanolic extract of Tephrosia purpurea was found to be 53.37 as compared to ascorbic acid with 17.68 IC 50value. All the treatment groups produced a significant decrease in serum TC, TG, HDL-C, and VLDL-C levels. In addition to the above, the serum LDL-C levels were significantly decreased by the Tephrosia purpurea extracts. The Tephrosia purpurea leaves extract significantly increased fecal cholesterol excretion on dose dependant manner. Thus, from the results it can be concluded that the methanolic extract of Tephrosia purpurea exhibit potent anti hyperlipedemic activity.

Keywords: Tephrosia purpurea, Anti -Hyperlipidaemic, Medicinal plants, TG, HDL, Cholesterol