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PARACAT
Published December 20, 2022 | Version V1
Dataset Open

Molecular mechanism for the synchronized electrostatic coacervation and co-aggregation of alpha-synuclein and tau

Creators

  • 1. Institute for Biocomputation and Physics of Complex Systems (BIFI), University of Zaragoza, 50018 Zaragoza, Spain
  • 2. Department of Condensed Matter Physics, Faculty of Sciences, University of Zaragoza, 50009 Zaragoza, Spain
  • 3. "Rocasolano" Institute for Physical Chemistry, CSIC, Serrano 119, Madrid E-28006, Spain

Description

The following metadata refers exclusively to electron paramagnetic resonance (EPR) measurements, which represent the contribution of the PARACAT students to this work

  • Data type: EPR spectroscopic measurements and simulations
  • Files are in .DTA, .DSC, .m, .mat, and .xlxs, formats
  • Information on origin of the data:
    • EPR spectroscopic measurements in .DTA and .DSC formats
    • EPR spectroscopic simulation and analyses in .m and .mat format
    • “Ready-to-plot”, processed EPR spectra are in .xlxs format.
  • The data are generated by:
    • CW-EPR measurements were performed with a Bruker ELEXSYS E580 X-band spectrometer equipped with a Bruker ER4118 SPT-N1 resonator operating at a microwave (MW) frequency of ∼9.7 GHz. The temperature was set to 25 °C and controlled by a liquid nitrogen cryostat.

 

  • If the dataset includes multiple files that relate to each other:
    • Files in PARACAT_WP3_20221219_EPR folder includes EPR spectroscopic measurements and computer simulations/analyses, original data are in  .DTA/.DSC formats; files in .m format were used to process the data.

NB. See the “READ ME” text file for more detailed information on files organization.

 

  • Information on:
    • Abbreviations:
      • avg = averaged
      • aS_24 = alpha-synuclein protein with TEMPOL spin label at position 24 of the polypeptidic chain
      • aS_122 = alpha-synuclein protein with TEMPOL spin label at position 122 of the polypeptidic chain
      • pLK = poly-lysine
      • Tau441 = Tau protein with complete amino-acid sequence
      • Tau_DNt = truncated Tau protein lacking N-terminal (see paper methods for further details)
    • Units of measurement:
      • Temperature: °C (Celsius)
      • Microwave Frequency: GHz (Giga-Hertz), MHz (Mega-Hertz), kHz (kilo-Hertz)
      • Microwave Power: mW (milli-Watt)
      • Magnetic Field: mT (milli-Tesla)
      • Time: s (seconds)
      • Concentration: μM (micro-Molar), % w/v (percentage weight-volume)

Notes

 EPR data: The .DTA and .DSC files can be opened on proprietary EPR softwares from Bruker or MatLab (version R2021b, used by the authors) with EasySpin toolbox (version 6.0.0-dev.34, used by the authors). The simulation scripts of EPR measurements can be opened and run on MatLab (version R2021b, used by the authors) with EasySpin toolbox (version 6.0.0-dev.34, used by the authors). MatLab can be purchased/downloaded from: https://www.mathworks.com/products/matlab.html; EasySpin toolbox can be downloaded from: https://easyspin.org/  "Ready-to-plot", processed EPR spectra can be opened with Microsoft Excel (or an analogous open access software).

Files

PARACAT_WP3_20221219_EPR.zip

Files (10.6 MB)

Name Size Download all
md5:cafca04a6b47fae78333f4ab8d6d59e2
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Additional details

Related works

Is supplement to
10.1038/s41467-022-32350-9 (DOI)

Funding

PARACAT – Paramagnetic Species in Catalysis Research. A Unified Approach Towards Heterogeneous, Homogeneous and Enzyme Catalysis 813209
European Commission