Published December 19, 2022 | Version v1
Journal article Open

Glycoproteomics of a single protein: revealing tens of thousands of Myozyme® glycoforms by hybrid MS approaches

Authors/Creators

  • 1. Department of Biosciences and Medical Biology, Bioanalytical Research Labs, University of Salzburg, Hellbrunner Straße 34, 5020 Salzburg, Austria
  • 2. Center for Proteomics and Metabolomics, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands
  • 3. Department of Biosciences and Medical Biology, Computational Systems Biology Group, University of Salzburg, Hellbrunner Straße 34, 5020 Salzburg, Austria
  • 4. Research Institute for Chromatography (RIC), President Kennedypark 26, 8500 Kortrijk, Belgium

Description

The folder related to the paper " Glycoproteomics of a single protein: revealing tens of thousands of Myozyme® glycoforms by hybrid MS approaches" by Di Marco et al. contains the following 9 raw files, 3 excel files and 4 Byonic files (2 byrslt and 2 byspec2):

Raw files:

  1. PGC-HPLC-MS/MS analysis of Myozyme® released N-glycans (File name: PGC_HPLC_MS_MS Released N_glycans Myozyme.mzML)
  2. Triplicates of nano RP-HPLC-MS/MS analyses of Myozyme® glycopeptides (File name: nano RP_HPLC_MS_MS Myozyme glycopeptide 1, 2 and 3.raw)
  3. micro RP-HPLC-MS/MS analyses of Myozyme® deglycosylated peptides by PNGase F (File name: micro RP_HPLC_MS_MS Myozyme PNGase F peptide not alk cysteinylation.raw)
  4. RP-HPLC-MS analysis deglycosylated intact Myozyme® by PNGase F in non-reducing condition (File name: RP_HPLC_MS PNGase F Myozyme non-reduced.raw)
  5. RP-HPLC-MS analysis deglycosylated intact Myozyme® by PNGase F in reducing condition (File name: RP_HPLC_MS PNGase F Myozyme reduced.raw)
  6. SAX-HPLC-MS analysis of intact Myozyme® (File name: SAX_HPLC_MS intact Myozyme.raw)
  7. SAX-HPLC-MS analysis of desialylated intact Myozyme® by sialidase (File name: SAX_HPLC_MS sialidase intact Myozyme.raw)

Excel files:

  1. Results of Byonic glycopeptide search (File name: Byonic search results Myozyme glycopeptides.xlsx)
  2. Results of Byonic PNGase F peptides, not alkylated, cysteinylated (File name: Byonic search results Myozyme PNGase F peptides not alk cysteinylation.xlsx
  3. Report of Skyline with the results of glycopeptide EICCs integration

Byonic files:

  1. Results of Byonic glycopeptide search (File name: Byonic search results Myozyme glycopeptides.byrslt and Byonic search results Myozyme glycopeptides.byspec2)
  2. Results of Byonic PNGase F peptides, not alkylated, cysteinylated (File name: Byonic search results Myozyme PNGase F peptides not alk cysteinylation.byrslt and Byonic search results Myozyme PNGase F peptides not alk cysteinylation.byspec2)

Files

Files (3.3 GB)

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