Investigation Of Genetic Diversity Of Chickpea Plants With ISSR And RAPD Markers

Genetic polymorphism of 62 chickpea samples have been studied using 15 ISSR and 30 RAPD markers. The avearge levels of polymorphism for ISSR and RAPD were found to be 98% and 80%, respectively. An average value for the genetic similarity index based on 2 markers was 0.65, the samples of cluster analysis with genetic similarity index within the limits of 0.27 to 1 were divided all genotypes into 12 main clusters. The mean values of Nei’s genetic diversity index (0.85 and 0.73 for RAPD and ISSR markers, respectively) shows, that both markers are effective for identification of chickpea genotypes. According to results of study, OPD 02, OPD11, OPD 09, OPD 4, OPF 03 and OPG 4 primers of RAPD markers, UBC 808 and UBC 827 primers of ISSR markers appeared to be most effective. UBC 880 primer of ISSR and SCAR primer (sequence characterized amplified region) can be used in the screening of drought tolerant genotypes of chickpea.