SYNTHESIS, CHARACTERIZATION AND ANTIMICROBIAL EVALUATION OF SOME PYRIMIDINE DERIVATIVES

The main objective of present work is to synthesis, characterization, and evaluation of antimicrobial activity of some pyrimidine derivatives containing O, N, and S in the ring. Pyrimidine derivatives were prepared in three steps. In the initial step, chalcones containing -NO₂ functional group were synthesized using Claisen Schmidt condensation of aromatic aldehydes with 2-acetyl pyridine/3-acetylpyridine in methanol in the presence of aqueous NaOH. In the next step, -NO₂ group was reduced to -NH₂ group. Resulting compounds containing NH₂ functional group were reacted with different dichlorothienopyrimidines and dichlorofuropyrimidines in the presence of N,N-diisopropylethylamine to obtain pyrimidine derivatives. Antibacterial and antifungal activity of pyrimidine derivatives were studied in vitro. Pyrimidine derivatives were synthesized and purified using column chromatography. Purity of synthesized pyrimidines was determined by HPLC. Pyrimidines were characterized by different analysis such as elemental analysis, infrared, nuclear magnetic resonance, and mass spectral analysis. Analytical data of synthesized pyrimidines indicates the proposed structures. Finally antibacterial and antifungal activity were observed in the synthesized pyrimidine derivatives.

Keywords: Antibacterial, antifungal, chromatography, spectral, medicines