Aurora A and cortical flows promote polarization and cytokinesis by inducing asymmetric ECT-2 accumulation - Data Archive

This archive contains all the images and raw data generated for: Katrina M Longhini & Michael Glotzer, “Aurora A and cortical flows promote polarization and cytokinesis by inducing asymmetric ECT-2 accumulation”

The data is organized by figure then experiment.  Each embryo folder contains formatted embryos, which are rotated, cropped, and photobleach corrected. Each recording starts at time 0 on the respective graph.

The “AccumulationDataNew” files contain all the data generated from  the “3pixel_membranefinder_w_dir.ijm” and “membrane_ROI_TimeSeries_max_scaled_dirs.ijm” scripts organized in a Tidy format generated in R using the first portion of the script “Edited_Membrane_Accumulation_Line_Script.Rmd”. These Fiji and R Markdown scripts are located in the “Analysis Scripts“ folder.

All data visualization scripts are included in the “Analysis Scripts” folder.

Sequences of plasmids generated for this paper are located in the “Plasmid Sequences” folder.