Synthesis of a molecularly imprinted polymers for selective extraction followed by the solid phase determination of Glibenclamide in pharmaceutical preparation and in human serum

   Abstract

    This paper demonstrates that the synthesising and storage of molecular-imprinted polymers (MIP) at room temperature using bulk polymerisation of Glibenclamide(Glib) is characterised by high sensitivity, reduced costs, increased stability, and extended life. The research used 1:10:20 mmol ratios of template, monomer, and cross-linking agents for the polymerisation in order to ensure an appropriate adsorption capacity. Benzoyl peroxide BPO was employed as the initiator for the functional monomer styrene C₈H₈, crosslinking Ethylene glycol dimethacrylate EGDMA C₁₀H₁₄O₄, thereby creating MIP for Glibenclamide (Glib-MIP) that could be characterised with UV-Visible Spectrophotometry at 274 nm for pharmaceutical drugs. Fourier-transform infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM) was used for the human serum. The elution process that was applied to the template (Glib) from the Glib-MIP created cavities that were caused by the porogenic mixture solvents that were created from methanol, chloroform, and acetic acid (70:20:10). The maximum adsorption capacity was 3.9523 µmol/g for 0.1g of Glib-MIP, which adhered to the Langmuir isotherm model. Hence, the template to monomer ratio was 1:1. A solid-phase extraction (SPE) syringe packed with molecular imprinted polymers (MIPs) was employed to selectively separate and pre-concentrate the Glibenclamide in multiple pharmaceutical drugs from several sources. The human serum was based on the use of deionized water to dilute the serum, followed by the heating of the serum with methanol. Subsequently, a few drops hydrochloric acid 1M were applied to detect Glibenclamide at UV region 274nm by applying the standard addition method.