Optimization of culture conditions for higher production of antimicrobial compounds by AZ-130 bacterial strain isolated from soil of Azerbaijan

AZ-130 bacterial strain was isolated from soil sample collected from Azerbaijan in 2014. After preliminarily culture and supernatant screenings for novel antibacterial compounds, AZ-130 showed strong gram-positive activity against pathogenic Staphylococcus aureus and Enterococcus faecalis strains. Based on range of its activity, AZ-130 strain that produces AZ-130 antibacterial compound was selected for the further characterization. The main goal of this study is to optimize growth conditions for AZ-130 to determine the optimal medium, incubation temperature and time point in which the production of the antimicrobial compound is highest. To achieve this goal, 4 different media types at 4 different temperatures, in total 16 different growth conditions were tested. Supernatants were collected and clarified at day 1, 2 and 3 or 5. All collected supernatants were analyzed by spot test and broth microdilution method against S.aureus. According to the spot test and broth microdilution results, AZ-130 produces the most antimicrobial compound in TB + 2% Glucose medium at 32°C; incubation time is 2 days.