Genetic variability of populations of Nilaparvata lugens stal (Delphacidae: Hemiptera) as revealed by random amplified polymorphic DNA

ABSTRACT

The Brown Plant Hoppers (BPHs) is one of the most serious insect pests in Asia where rice is widely planted. It is a typical vascular feeder and considered one of the most serious pests of all rice herbivores. It causes reduction in leaf area, photosynthetic rate, nitrogen concentrations of leaf and stem, chlorophyll contents and organic dry weight. It also causes high infestation ratios, reduction in egg numbers and survival ratios of eggs and nymphs on susceptible rice plants. Besides it also transmits several viral diseases that cause crop damage .However, BPH has dramatically emerged as a major pest causing massive losses in tropical rice production under the “Green Revolution” with high yielding IR varieties. In 2005, India reported a loss of 2.7 million tons of rice due to direct damage. About twelve populations of N.lugens were collected from different localities of Tamilnadu from the rice field and were subjected to RAPD analysis using twenty random decameric primers.  Out of the twenty RAPD primers tested, three primers have produced clear, consistent and reproducible RAPD bands for all the populations. UPGMA based dendrogram analysis grouped all the populations of N.lugens into many clusters based on the genetic similarity coefficient. 

KEYWORDS: Brown Plant Hopper, N.lugens, Green revolution, RAPD, UPGMA, Dendrogram,  Genetic Similarity

REFERENCE

1. Saldanha LA. 2000. Genetic variation of the European corn borer Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae). PhD Dissertation. University of Nebraska.
2. Pornkulwat S, Skoda SR, Thomas GD, Foster JE. 1998. Random AmplifiedPolymorphic DNA used to identify genetic variation in ecotypes of the European corn borer (Lepidoptera: Pyralidae). Annals of the Entomological Society of America 95: 719-725.
3. Black WC, Duteau NM, Puterka GJ, Nichols JR, Pettorini JM (1992). Use of the random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) to detect DNA polymorphisms in aphids (Homoptera: Aphididae). Bull. Entomol. Res. 82: 151-159.
4. Haymer DS, Mclnnis DD (1994). Resolutions of populations of the Mediterranean fruitfly at the DNA level using random primers for the polymerase chain reaction, Genome 37: 244-248.
5. Kambhampati S, Black WC, Rai KS (1992). Random amplified polymorphic DNA of mosquito species and populations (Diptera: Culicidae): Techniques, statiscal analysis and applications. J. Med. Entomol. 29: 939-945.
6. Mendel Z, Nestel D, Gafny R (1994). Examination of the origin of the Israeli population of Matsucoccus josephi (Homoptera:Matsucoccidae) using random amplified polymorphic DNApolymerase chain reaction method. Ann. Entomol. Soc. Am. 87(2): 165-169.
7. Puterka GJ, Black IV, Steiner WC, Burton RL (1993). Genetic variation and phylogenetic relationships among worldwide collections of the Russian wheat aphid, Diuraphis noxia (Mordivilko), inferred from allozyme and RAPD-PCR markers. Heredity 70: 604-618.
8. Dowdy AK, McGaughey WH (1996). Using random amplified polymorphic DNA to differentiate strains of the Indianmeal moth (Lepidoptera: Pyralidae). Environ. Entomol. 25(2): 396-400.
9. Yang ZF, Zhang F, He Q, He GC. 2005. Molecular dynamics of detoxification and toxin-tolerance genes in brown planthopper (Nilaparvata lugens Stål, Homoptera: Delphacidae) feeding on resistant rice plants. Arch. Insect Biochem. Physiol. 59:59-66.
10. Yin JL, Xu HW, Wu JC, Hu JH, Yang GQ. 2008. Cultivar and insecticide applications affect the physiological development of the brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae). Environ. Entomol. 37:206-212.
11. Hassan M.E and Biswas B, 2013. First record of Courtesius illuminates distant (Hemiptera: Pyrrhocoridae) from India. Biolife 2013; 1(3); 93-95.
12. Liu ZW, Williamson MS, Lansdell SJ, Denholm I, Han ZJ, Millar NS. 2005. A nicotinic acetylcholine receptor mutation conferring target-site resistance to imidacloprid in Nilaparvatalugens (brown planthopper). Proc. Natl. Acad. Sci. USA 102:8420-8425.
13. Denno RF, Olmstead KL, McCloud ES. 1989. Reproductive cost of flight capability: a comparison of life-history traits in wing dimorphic planthoppers. Ecol. Entomol. 14:31-44.
14. Claridge MF, den Hollander J. 1980. The biotypes of the rice brown planthopper, Nilaparvata lugens. Entomol. Exp. Appl. 27:23-30.
15. Pathak PK, Heinrichs EA. 1982. Selection of biotype population-2 and population-3 of Nilaparvata lugens (Homoptera, Delphacidae) by exposure to resistant rice varieties. Environ. Entomol. 11:85-90.
16. Cagampang, G. B., Pathak, M. D., Juliano, B. O (1974). Metabolic changes in the rice plant during infestation by the brown plant hopper, Nilaparvata lugens Stal (Hemiptera: Delphacidae). Appl. Entomol. Zool. 19, 174–184.
17. Sogawa, K (1982). The rice brown plant hopper: feeding physiology and host plant interactions. Annu. Rev. Entomol. 27, 49–73.
18. Rivera, C. T., Ou, S. H., Iida, T. T (1966). Grassy stunt disease of rice and its transmission by the plant hopper Nilaparvata lugens Stal. Plant Dis. Rep. 50,453–456.
19. Huang, Z., He, G., Shu, L., Li, X and Zhang, Q. (2001). Identification and mapping of two brown planthopper resistance genes in rice. Theor. Appl. Genet. 102:929–934.
20. Kay, H.E., Coutinho, H.L.C., Fattori, M., Manfio, G.P., Goodacre, R., Nuti, M.P., Basaglia, M. and Beringer, J.E. 1994. The identification of Bradyrhizobium japonicum strains isolated from Italian soils. Microbiology. 194: 2333-2339.
21. Kosier, B., Puhler, A and Simon, R (1993). Monitoring the diversity of rhizobium meliloti field and microorganism isolates with a novel rapid genotyping method using insertion elements. Mol. Ecol. 2 : 35-46.
22. Roderick, G.K. (1996) Geographic structure of insect population: gene flow, phylogeography, and their uses. Annual Review of Entomology 41, 263–290.
23. Simon, C., Prati, F., Beckenbach, A., Crespi, B., Liu, H. & Flook, P. (1994) Evolution, eighting, and phylogenetic utility of 252 J.H. Mun et al. mitochondrial gene severances and a compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America 87, 651–701.
24. Srinivas Gidhamaari, Boominathan and Estari Mamidala. (2012). Studies of efficiency of immobilized bacteria in tannery effluent treatment. Journal of Bioinnovation, 2(2), 33-42.
25. Hoshizaki, S. (1994) Detection of isozyme polymorphism and estimation of geographic variation in the brown plant hopper, Nilaparvata lugens (Stål) (Homoptera: Delphacidae). Bulletin of Entomological Research 84, 502–508.
26. Demayo, C.G., Saxena, R.C. & Barrion, A.A. (1990) Allozyme variation in local population of the brown plant hopper Nilaparvata lugens (Stål) in the Philippines. Philippine Entomology 8, 737–748.
27. Kazushige, S., Juan, Z and Hai, Q.Z. (2003). Applications of DNA-Markers to analyze Rice Plant hopper Resistance genes. Chinese J. Rice Sci. 17: 37-46.
28. Maniatis, T., E.F. Fritsch and J. Sambrook (1982). Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA.
29. NEI, M. 1978.  Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics. 89: 583-590.
30. Sambrook, J., Fritsch, E.F. and Maniatis, T. 1989. Molecular cloning: a laboratory manual, 2^nd ed. Cold spring harbor laboratory press, cold spring harbor, New York.
31. Joshi, S.P., Gupta, V.S., Aggarwal, R.K., Ranjekar, P.K. and Brar, D.S. (2000). Genetic diversity and phylogenetic relationship as revealed by inter sample sequence repeat (ISSR) polymorphism in the genus Oryza. Theor. Appl. Genet. 100: 1311-1320.
32. Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. and Tingey, S.V (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18 : 6531-6535.