PEROXIDASE ISOENZYME POLYMORPHISM IN THE GENUS PRUNUS, SUBGENUS CERASUS

The polymorphism of peroxidase was studied in 31 cherry accessions, representing six following species Prunus cerasus, Prunus avium, Prunus fruticosa, Prunus mahaleb, Prunus serrulata, Prunus gondouinii and two widely-used standard cherry rootstocks ‘Gisela 5’ and ‘Colt’. Six ‘Oblačinska’sour cherries, four wild sweet cherries, five ground and one mahaleb genotypes were selected from the natural populations of Serbia. Inner barks from one-year-old shoots and young actively growing leaves were used for protein extraction. The polymorphism of peroxidase was obtained both for leaf and inner bark tissues. The analysis of the leaf material showed the unique zymograms for all six species and two interspecies hybrids. Higher numbers of polymorphic loci and banding patterns were detected when protein was extracted from the leaves, than from inner bark. Obtained results indicate that
the polymorphism determination of genus Prunus, subgenus Cerasus can be done on the basis of peroxidase, but it would not be useful for discrimination of different genotypes and clones.