THE EFFECT OF BOVINE SERUM ALBUMIN IN CULTURE MEDIA ON BOVINE SPERMATOZOA MOTILITY PARAMETERS IN VITRO

The aim of this study was to analyze the influence of bovine serum albumin (BSA) used in different culture media on motility and viability of bovine spermatozoa during a 24-hour in vitro cultivation. We compared native samples (N) and samples cultivated in a commercial egg yolk medium (R) with samples cultivated in several experimental media containing 10% or 20% BSA. The analysis was carried out during three time periods (time 0, time 1 hour and time 24 hours) using Sperm VisionTM CASA system. Our study shows an obvious time-dependent decrease of the spermatozoa viability parameters in all experimental groups cultured for 24 hours. The lowest spermatozoa motility parameters were detected in native sample. Culture media with 10% BSA content showed only average quality and unsatisfactory results. Overall best motility parameters were detected when applying 20% BSA, especially in the medium D containig triladyl, 20% BSA and 5% glucose. Comparing results detected in this group with the results from the commercial egg yolk medium significant (P<0.01) differences for progressive motility, as well as for distance average path (P<0.001), velocity average path (P<0.001) and amplitude of lateral head displacement (P<0.001) were detected. This study demonstrates
that BSA could be a good protein suplement for long-term bovine spermatozoa cultivation and further processing.