Methane-related community of a carbonate-enriched pockmark, Brazilian Southeastern continental slope

Supplementary Material of the article "Methane-related community of a carbonate-enriched pockmark, Brazilian Southeastern continental slope"

- SF1. Sediment sampling in Box-corer, presenting the core positioning and sediment surface color

- SM1. Organic compounds - Analytical procedures

- SM2. Sediment dark carbon fixation (DCF) and Heterotrophic Microbial Production (HMP) - Analytical procedures

-  SF2. Rarefaction curve

- SM3. Methodology details for the functional prediction database

- ST1. ASVs alpha diversity indexes grouped by enrichment type and incubation time

-  SF3. Microbial community composition of deep-sea sediments of a pockmark of the Brazilian Southeastern Atlantic enriched with methane. Sequences were taxonomically classified using the Silva database v. 138

-  SF4. Upset Plot composed by bacterial and archaeal ASVs detected on the harvesting times of the methane incubation of deep-sea intact pockmark sediment cores of Brazilian SE Atlantic. Black lines connecting circles indicate shared taxonomies. Black points solely indicate unique taxonomy presence per set. Vertical  bars  indicate  intersection  size (number  of  complete taxonomies, up to Species from the assignment with Silva v.138)  on  each  set.  Datasets: Control_16h, cores without CH₄ input harvested after 16h; Control_940h, core without CH₄ input harvested on 40th day; Enriched_16h, core with CH₄ input harvested after 16h; Enriched_120h, core with CH₄ input harvested on the 5th day; Enriched_240h, core with CH₄ input harvested on the 10th day; Enriched_720h, core with CH₄ input harvested on the 30th day; Enriched_960h, core with CH₄ input harvested on the 40th day.

- SF5. Methane-enriched differentially abundant taxa recovered with ANCOM statistics