Phytochemical analysis & in-vitro cytogenetics assay of chromosomal aberration on peripheral human blood lymphocytes by the leaf crude extract- Passiflora foetida L

ABSTRACT

The present investigation is to evaluate the analysis preliminary photochemical screening and In-vitro cytogenetic assay measuring chromosomal aberration frequencies induced by the leaf crude extract Passiflora  foetida L. as an active ingredient in peripheral human blood lymphocyte. Whole blood lymphocytes were cultured in-vitro using a standard protocol and the treatment with the leaf crude extract. The plant extract and fractions revealed preliminary phytochemical compounds with no toxicity. The present investigation revealed that no chromosomal aberration was found in the test sample. And also this study supports, the traditional medicines (herbal extracts) to cure many diseases like diarrhea, intestinal tract, throat, ear infections, fever and skin diseases. The toxicological data are used to evaluate the safety and efficacy of new chemical entities (NCE). One of the most important aspects of safety pharmacology is the evaluation of genotoxicity potential of the NCE. Genotoxicity studies are conducted both at non-clinical and clinical levels.

Keywords: Passiflora foetida L., Methanol extracts, Genotoxicity, RPMI-60, Mytomycine C, Chromosomal Aberration.

BIBLIOGRAPHY

1. Akiyama H, Fujii K, Yamasaki O, Oono O and Iwatsuki K “Antibacterial action of several tannins against Staphylococcus aureus"J. Antimicrob. Chemother.2001; 48(4): 487–91.
2. Asadujjaman M, Mishuk AU, Hossain MA, Karmakar UK. Medicinal potential of Passiflora foetida L. plant extracts: biological and pharmacological activities. J Integr Med. 2014; 12(2): 121-126.
3. Ashok J. Patil. (2014). Giemsa Banding In Male Rat (Rattus Rattus). Biolife. 2(3):842-844.
4. Babu.P. V. A, Liu.D, Gilbert.E.R. (2013). "Recent advances in understanding the anti-diabetic actions of dietary flavonoids". The Journal of Nutritional Biochemistry 24 (11): 1777–1789. doi:10.1016/j.jnutbio.2013.06.003. PMC 3821977. PMID 24029069.
5. Balasubramaniam A, Manivannan.R, Baby.E, 2010, Int. J. Drug Formulation Res., 1(II): 144-154.
6. Bandele OJ, Clawson SJ, Osheroff N (2008). "Dietary polyphenols as topoisomerase II poisons: B-ring substituents determine the mechanism of enzyme-mediated DNA cleavage enhancement".Chemical Research in Toxicology 21 (6): 1253–1260. doi:10.1021/tx8000785. PMC 2737509. PMID 18461976
7. Barjesteh van Waalwijk van Doorn-Khosrovani S, Janssen J, Maas LM, Godschalk RW, Nijhuis JG, van Schooten FJ (2007). "Dietary flavonoids induce MLL translocations in primary human CD34+ cells". Carcinogenesis 28 (8): 1703–9. doi:10.1093/carcin/bgm102. PMID 17468513
8. Bleu G.M., Kouakou K, Zahoui O. S, Touré Alassane, Traoré Flavien. Oral acute toxicity and estrogenic effects of the extracts of Passiflora foetida Linn. (Passifloraceae) leaves in female Wistar albino rats. Annals of Biological Research, 2012, 3 (9):4609-4616.
9. Brogger A, Hogstedt B, Knudsen l, Lambert B, Linnainmaak Mitelanan G, Norsdenson I, Reutewall C, Salonaa S, Skerfiving S, Sorsa M 1990. An inter nordic prospective study on cytogenetic end points and cancer risk. Cancer Genet. Cytogenet, 45:85-92
10. Cazarolli LH, Zanatta L, Alberton EH, Figueiredo MS, Folador P, Damazio RG, Pizzolatti MG, Silva FR (2008). "Flavonoids: Prospective Drug Candidates". Mini-Reviews in Medicinal Chemistry 8 (13): 1429–1440.
11. Esselen M, Fritz J, Hutter M, Marko D (2009). "Delphinidin Modulates the DNA-Damaging Properties of Topoisomerase II Poisons". Chemical Research in Toxicology 22 (3): 554–64. doi:10.1021/tx800293v. PMID 19182879.
12. Ferretti. G, Bacchetti. T, Masciangelo. S, Saturni.L. (2012). "Celiac Disease, Inflammation and Oxidative Damage: A Nutrigenetic Approach". Nutrients 4 (12): 243–257. doi:10.3390/nu4040243. PMC 3347005. PMID 22606367. 
13. Friedman M (2007). "Overview of antibacterial, antitoxin, antiviral, and antifungal activities of tea flavonoids and teas". Molecular Nutrition and Food Research 51 (1): 116–134. doi:10.1002/mnfr.200600173. PMID 17195249.
14. Kellen.J.A., 1996, Tamoxifen: Beyond the antiestrogen. BirkhäuserVerlag, Berlin, pp.392.
15. Kolodziej H and Kiderlen  AF.Antileishmanial activity and immune modulatory effects of tannins and related compounds on Leishmania parasitised RAW 264.7 cells". Phytochemistry. 2005; 66(17): 2056–71.
16. Lu L, Liu SW, Jiang SB and Wu SG. Tannin inhibits HIV-1 entry by targeting gp41. Acta Pharmacol. Sin.2004; 25(2): 213–8.
17. Manach.C, Mazur.A, Scalbert.A (2005). "Polyphenols and prevention of cardiovascular diseases". Current opinion in lipidology 16 (1): 77–84.doi:10.1097/00041433-200502000-00013. PMID 15650567.
18. Manner S, Skogman M, Goeres D, Vuorela P, Fallarero A (2013). "Systematic exploration of natural and synthetic flavonoids for the inhibition of Staphylococcus aureus biofilms". International Journal of Molecular Sciences 14 (10): 19434–19451.doi:10.3390/ijms141019434.PMC 3821565. PMID 24071942.
19. Mehta Kavit, Patel B.N.and Jain B.K. 2013. Phytochemical analysis of leaf extract of Phyllanthus fraternus. Research Journal of Recent Sciences. ISSN 2277-2502 Vol. 2 (ISC-2012), 12-15.
20. Mehtakavit, Patel B.N. and Jain B.K.. 2013. Phytochemical analysis of leaf extract of Phyllanthus fraternus. ISSN 2277-2502.
21. Nobre-Junior and Helio V.”Neuroprotective Actions of Tannins from Myracrodruon urundeuva on 6-Hydroxydopamine-Induced Neuronal Cell Death ".Journal of Herbs, Spices & Medicinal Plants. 2007; 13(2).
22. Perumal Samy R.and Gopala Krishnakone P., 2007. Current status of herbal and their future perspectives,Nature.Proceedings:hdl:10101/npre.1176:1.
23. Ramakrishnan. V, Kumar. S. G and Govindaraju.S. (2011), Cytogenetic analysis of micronuclei, sister chromatid exchange and chromosomal aberrations in pan masala chewers. ISSN-0975-2699.
24. Rasool.S.N, Jaheerunnisa.S, Jayaveera.K.N, Kumar.S.K.. 2011. Int. J. Applied Res. Nat. Prod., 4(2): 24-31.
25. Schorderet M (1992). Pharmacologie. Des concepts fondamentaux aux applications theurapeutiques. Editions Slatkine Gen eve, Edition Frison-Roche paris pp. 33-34.
26. Sousa RR, Queiroz KC, Souza AC, Gurgueira SA, Augusto AC, Miranda MA, Peppelenbosch MP, Ferreira CV, Aoyama H. (2007). "Phosphoprotein levels, MAPK activities and NFkappaB expression are affected by fisetin". J Enzyme Inhib Med Chem 22 (4): 439–444. doi:10.1080/14756360601162063. PMID 17847710.
27. Souza SMC, Aquino LC, Milach Jr AC, Bandeira MA Nobre ME and Viana GS. "Antiinflammatory and antiulcer properties of tannins from Myracrodruon urundeuva Allemão (Anacardiaceae) in Rodents".Phytotherapy Research. 2006; 21(3): 220–225.
28. Venkatasubbaiah, K., Divya, K., Pallavi, C2 ., Kamala, K, Swamy, MV., Rao, KJ and Thyaga Raju, K. (2013). Analysis Of Micronuclei Formation Is An Indicator Of GenotoxicityAssessment To Acrylamide In Rats And Developing Chick Embryos. Biolife. 1(2):-35-44.
29. Yadav JS, Thakur S 2000. Cytogenetic damage in bidi smokers. Nicotine and Tobacco Res, 2: 97-103.
30. Yamamoto, Yumi; Gaynor, Richard B. (2001). "Therapeutic potential of inhibition of the NF-κB pathway in the treatment of inflammation and cancer". Journal of Clinical Investigation 107 (2): 135–42.doi:10.1172/JCI11914. PMC 199180. PMID 11160126.