EVALUATION OF ANTIBIOTIC RESISTANCE IN E.COLI STRAINS FROM UTI CLINICAL ISOLATES

ABSTRACT

Antibiotic resistance among pathogens causing urinary tract infection (UTI) is increasing at an alarming rate. E.coli is found to be the most prevalent among the common bacterial uropathogens isolated. The present study was undertaken to evaluate current antibiotic resistance pattern among the multi drug resistant strains of Escherichia coli isolated from positive UTI samples. 342 clean catch midstream urine samples were collected and processed using standard methods on Blood agar, Mac-Conkey agar and EMB agar. A total of 138 urine samples were reported positive for E.coli which were further characterized by using Morphological and Biochemical characterization. Antimicrobial susceptibility determined by Kirby Bauer disc diffusion method as per Clinical and Laboratory Standard Institute (CLSI) guideline showed 62 isolates (44%) were multi drug resistant. Out of the 14 clinically prescribed antibiotics tested, high level of resistance was seen to Ciprofloxacin (75%), Gatifloxacin (68%), Ceftazidime (62%), Meropenem (51%) and Imipenem (39%). Nitrofurantoin (20%) showed lowest resistance rate respectively. Due to the high prevalence of drug resistance, regular monitoring of patterns of resistance in different areas is very much necessary. This would prevent the unsuitable consumption of the drugs, helps in implementing judicious measures for control of the infection and also deciding empirical therapies for preventing urinary tract infection.

Keywords: Escherichia coli, Antibiotic Drug Resistance, Urinary tract infection

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