Mouse SIRV and simulated data used in the IsoQuant publication

This dataset contain main simulated and SIRV sequencing data used in the IsoQuant publication.

NOTE: SIRVs BAM file contains ONT R9.4 data, R10.4 data that was used in the publication is available in the newer version.

- Reduced mouse GENCODE vM26 annotation: 15% of expressed isoforms removed, expression profile based on real mouse brain sequencing data; also contains GTF with expressed transcripts.

- Mouse PacBio simulated data: 6 million reads generated with IsoSeqSim, 1.4% error rate. Provided as a sorted BAM file, reads were mapped to GRCm39 genome using minimap v2.18.

- Mouse ONT R10.4 simulated data: 30 million read generated with NanoSim, 2.8% error rate. Provided as a sorted BAM file, reads were mapped to GRCm39 genome using minimap v2.18.

- Mouse ONT R9.4 simulated data: 30 million read generated with NanoSim, 15.9% error rate. Provided as a sorted BAM file, reads were mapped to GRCm39 genome using minimap v2.18.

- Lexogen SIRV Set4 sequenced on ONT MinIon with R9.4 chemistry, filtered >=Q12. Provided as a sorted BAM file, reads were mapped to SIRV reference using minimap v2.18. NOTE: the file name Lexogen.SIRVs.Set4.ONT_cDNA.R10.4.HQ.sorted.bam is incorrect and contains R9.4 data (not used in the publication).

- SIRV reference genome and reduced annotation, also available at Lexogen download page.