OME-Zarr 3D hiPSCs with labels & measurements, 2x2 field of views
Creators
Description
These are 2 small OME-Zarr files of the data from 10.5281/zenodo.7057076.
They have been processed using fractal-client 0.2.1, fractal-server 0.1.4 and fractal-tasks-core 0.1.9 using this workflow: https://github.com/fractal-analytics-platform/fractal/tree/main/examples/08_cardio_2x2_dataset_processing_zenodo
Both Zarr files are Zip-compressed to allow easier upload & download from Zenodo.
20200812-CardiomyocyteDifferentiation14-Cycle1.zarr contains 3 3D channels and a table with regions of interest for the 4 field of views contained in this data.
20200812-CardiomyocyteDifferentiation14-Cycle1_mip.zarr contains the same 3 channels, but as maximum intensity projections. It contains nuclear segmentation through cellpose. It also contains 2 tables: The region of interests like in the 3D data, as well as measurements performed with napari-skimage-regionprops.
The tables are stored in the OME-Zarr file according to the proposed OME-NGFF table spec in AnnData.
The 3 channels are:
- 0: DAPI, nuclear stain
- 1: nanog, antibody staining with Bio-Techne AG, AF1997-SP, Lot KKJ0617121 for the stemness marker nanog
- 2: Lamin B1, antibody staining with Abcam, ab16048, Lot GR3244890-2 for the nuclear envelope marker Lamin B1
This updated version now passes the ngff schema validation. A version with 3D segmentation is available here: https://zenodo.org/record/7144919
Files
20200812-CardiomyocyteDifferentiation14-Cycle1.zarr.zip
Files
(903.0 MB)
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md5:e3128174971717f3597392c71c12c1e2
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811.7 MB | Preview Download |
md5:d8165bffced9d480285980829f3d40b8
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