VALITEST - D2.2 "Best practice" guidelines for validation and routine use of non-targeted techniques in diagnostic setting which could serve as a basis for a new EPPO Standard

These guidelines provide recommendations on the process of selection, development, validation and verification of high-throughput sequencing (HTS) tests and the quality assurance for their routine use as diagnostic tests in plant health laboratories. These guidelines are general to enable a broad application in all plant health fields with appropriate flexibility to account for the changes of technologies.

The guidelines cover all steps from sample collection to reporting including risk analysis, test selection/test development, test validation/verification, quality checks to ensure the validity of the results, confirmation of the detection and interpretation of biological relevance within a quality management system. Furthermore, these guidelines provide an overview of the HTS technologies and their applications in plant health.