Immunohistochemistry of Multimodal profiling of lung granulomas in macaques reveals cellular correlates of tuberculosis control

(A) Architecture of macaque TB lung granuloma, where lymphocytes and macrophages are present in distinct regions. Immunohistochemistry and confocal microscopy were performed on a granuloma from an animal at 11 weeks post-Mtb infection to visualize localization of CD11c+ macrophages (cyan), CD3+ T cells (yellow), and CD20+ B cells (magenta)

(B) Detection of mast cells in a 10-week NHP granuloma using immunohistochemistry, staining for tryptase (green) and c-kit (CD117)(red).

(C) Detection of mast cells in a human lung granuloma. Hematoxylin and eosin stain and immunohistochemistry with multinucleated giant cells (stars, (top left) and c-kit (CD117) staining (indicated by arrows, top and bottom right).