Identification and single-base gene-editing functional validation of a cis-EPO variant as a genetic proxy for EPO-increasing therapies.

Raw fast-qc files for RNA sequencing analysis of whole EPO gene knock-outs generated through CRISPR-Cas9 gene-editing in HEK-293 cells compared to wild-type controls. 

Wild-type cell-lines (Empty 1 - Empty 4) were transfected with empty CRISPR-Cas9 constructs to ensure cells were treated under the same experimental conditions. Whole EPO gene knock-outs were generated using a double gRNA approach with CRISPR-Cas9 gene-targeting. Two whole EPO gene knock-out cell-lines were generated (KO-A and KO-B).

Library preparation was performed using the TruSeq DNA HT Library Preparation Kit using the 3’ poly-A tail primer Oligo(dT) from Illumina (Illumina, California, USA). RNA Sequencing was performed using the Illumina HiSeq 2500 high-throughput sequencing system (Illumina, California, USA). We resulted in 75 bp paired-end sequences.