Published July 8, 2022 | Version version 1
Journal article Open

scm6A-seq reveals single-cell landscapes of the dynamic m6A during oocyte maturation and early embryonic development

  • 1. Beijing Institute of Genomics
  • 2. Beijing Institute of Geomics

Description

### The code is used in the linux system.
#Python environment and Cutadapt (version 2.10 or latest) needs to be installed before data processing.

Installation on a Debian-based Linux distribution (https://cutadapt.readthedocs.io/en/stable/installation.html)

 

################ If there are hunderds and more cells are in scm6A-seq data, tso-mix separation is needed.

sh ./scm6A-tsomix-separation.sh ${dir}/Test.fastq.gz

Expcted output file:
*index1.fastq
*index2.fastq
*index3.fastq
*N4.fastq

############## After tso-mix separation,cellular barcodes is needed for single cell reads isolation. Or if less than 20 cells in one library, single cell reads isolation is performed directlty.
### Test 10 thousand reads for single cell isolation with 6 cores, cost 1m24.507s

sh ./scm6A-SCbarcodes-isolation.sh

Expected output files:
*_${barcode}.fastq.gz

 

Files

barcode.txt

Files (326.5 kB)

Name Size Download all
md5:472c55ea734692ad6096be5e0a6293f0
160 Bytes Preview Download
md5:0cb32ceebaed65867d41d8d58d94fee8
1.8 kB Preview Download
md5:a47c7954158150b3908574f5edf24008
513 Bytes Download
md5:2386275af9601f42298b23c345a5037e
10.9 kB Download
md5:691fe1bc5c412240ee0ffc819da9008e
159.2 kB Download
md5:cb04083ed098981d568a6aa5cf4228bf
153.9 kB Download