Published July 2, 2022 | Version 0.0.1
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Pretrained nucleAIzer models for microscopy datasets

  • 1. Biological Research Centre, Szeged

Description

This upload consists of 4 models trained using the nucleAIzer algorithm (Hollandi et al. & https://github.com/spreka/biomagdsb) for segmenting the following microscopy image types (profiles):

  1. general nucleus
  2. fluorescent nucleus
  3. tiny&clumped fluorescent nucleus
  4. tissue nucleus
  5. fluorescent cytoplasm
  6. tissue nucleus IHC
  7. tissue nucleus H&E

Models for each type:

  1. mask_rcnn_general.h5
  2. mask_rcnn_general.h5
  3. mask_rcnn_general.h5
  4. mask_rcnn_general.h5
  5. mask_rcnn_cytoplasm.h5
  6. mask_rcnn_tissue_bluebrown.h5
  7. mask_rcnn_tissue_pink.h5

The above models were trained on resized images such that  the median nuclei diameter is 40 pixels.

Additionally, a presegmentation model is also attached (mask_rcnn_presegmentation.h5) for generating the weak labels on the test set (first step in the training pipeline), while the mask_rcnn_fluo_simple.h5 is a general model that can be used for automatic object size estimation.

We additionally attach a profiles.json file describing the parameters for the models for the above segmentation tasks that can be loaded by our prediction and training code used in the napari nucleAIzer plugin (https://pypi.org/project/napari-nucleaizer/ and https://pypi.org/project/nucleaizer-backend/).

If you use our models in your research work, please cite our original paper (Hollandi et al.: nucleAIzer: a parameter-free deep learning framework for nucleus segmentation using image style transfer, Cell Systems, 2020. https://doi.org/10.1016/j.cels.2020.04.003)

Files

fluorescent_cytoplasm.png

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Additional details

Related works

Is supplemented by
Journal article: 10.1016/j.cels.2020.04.003 (DOI)