Dataset Open Access
Covalent Data Screen of TBXTA-C006
Zachary Davis-Gilbert;
David H. Drewry;
Mohammad Anwar Hossain;
Joseph Newman;
Opher Gileadi;
Carrow I Wells;
Nergis Imprachim;
Angeline E Gavard
Uploaded data set contains the mass spec labelling data covalent inhibitor campaign against TBXTA-C006. The data was collected at Oxford and it contains the Structure IDs we use, the SMILE strings, and the %-labelling of protein by mass spec at 5 uM (10 minutes or 60 minutes) or 50 uM (60 minutes). A more detailed protocol is given below.
Protocol:
Protein is TBXTA-C006 (13 mg/ml 130x final concentration 0.1 mg/ml or 5 uM) Buffer is 10 mM Hepes pH 7.5, 150 mM NaCl, 0.5 mM TCEP (freshly prepared)
Compounds are in stock plates at 100 mM, Initial 1:40 dilution into buffer (50 uM) and further 1:10 dilution for Low conc (5uM)
1 ul of compound is added to 50 ul of protein for final 50 uM and 5 uM conc.
At 1hr (or less) 5ul is removed and added to 50 ul of MS buffer.
| Name | Size | |
|---|---|---|
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Covalent Summary Slide .xlsx
md5:f2d55e8e8dfe4347ba77b2a0ef721cd0 |
20.0 kB | Download |
| All versions | This version | |
|---|---|---|
| Views | 125 | 125 |
| Downloads | 20 | 20 |
| Data volume | 400.0 kB | 400.0 kB |
| Unique views | 105 | 105 |
| Unique downloads | 20 | 20 |
- Publication date:
- June 9, 2022
- DOI:
-
- Keyword(s):
- TBXT Covalent Ligand Brachyury
- License (for files):
- Creative Commons Attribution 4.0 International