Evaluation of Enzyme Producing K. Pneumoniae and Their Susceptibility to Other Anti-Biotics

Antimicrobial-resistant microbes are now infecting doctors, veterinarians, and other infection control specialists. Material & Method: Collection of samples and isolation of K. pneumoniae, Anti-microbial Susceptibility pattern, Hodge's modified test, Extraction of the bla KPC2 gene's DNA Result & Discussion: More than half of the isolates were resistant to both imipenem and meropenem, with four being just resistant to imipenem. Among the 38 carbapenem resistant isolates, 71% had imipenem MICs above CLSI norms, whereas the remaining 79.9% were intermediately resistant. The MIC50 and MIC90 were 0.19-32, 4 and 16 g/ml, respectively (21.05 percent ). Eight of the isolates had MHT. The blaKPC-2 imipenem MIC is 4-32 g/ml. Neither colistin nor co-trimaxazole resistant BlaKPC-2 isolates. Conclusion: Our institution found blaKPC-2 to be a carbapenem resistant K. pneumoniae pathway. Results demonstrated imipenem disc diffusion can quickly identify these isolates. These illnesses and their associated drug resistance demand infection control and antibiotic stewardship measures.