Dataset: Label-free detection of methicillin resistance in Staphylococcus aureus using different Raman-spectroscopy approaches
Authors/Creators
- 1. Leibniz Institute of Photonic Technology Jena, Albert-Einstein-Str. 9, 07745 Jena, Germany
- 2. Leibniz Institute of Photonic Technology Jena, Albert-Einstein-Str. 9, 07745 Jena, Germany, InfectoGnostics Research Campus Jena, Philosophenweg 7, 07743 Jena, Germany
- 3. Leibniz Institute of Photonic Technology Jena, Albert-Einstein-Str. 9, 07745 Jena, Germany, Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University, Lessingstraße 10, 07743 Jena, Germany
- 4. Leibniz Institute of Photonic Technology Jena, Albert-Einstein-Str. 9, 07745 Jena, Germany , InfectoGnostics Research Campus Jena, Philosophenweg 7, 07743 Jena, Germany, Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University, Lessingstraße 10, 07743 Jena, Germany
- 5. Leibniz Institute of Photonic Technology Jena, Albert-Einstein-Str. 9, 07745 Jena, Germany, InfectoGnostics Research Campus Jena, Philosophenweg 7, 07743 Jena, Germany, Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University, Lessingstraße 10, 07743 Jena, Germany, Jena Biophotonics and Imaging Laboratory, Albert-Einstein-Str. 9, 07745 Jena, Germany, Center for Sepsis Control and Care, Jena University Hospital, Am Klinikum 1, 07747 Jena, Germany
Description
This is the dataset accompanying the submission of the manuscript: Label-free detection of methicillin resistance in Staphylococcus aureus using different Raman-spectroscopy approaches in the journal Microbiology Spectrum.
The data description is the following:
Strains
16859MRSA= Strain AUSTR-07-16859 MRSA
16859MSSA= Strain AUSTR-07-16859 MSSA
CC8MRSA= Strain 08V15773
CC8MSSA= Strain MRSA2010-174
AUSTR05MRSA= Strain AUSTR-05-15441 MRSA
AUSTR05MSSA= Strain AUSTR-05-15441 MSSA
CC361MRSA= Strain UAE-Abu Dhabi-020
CC361MSSA= Strain UAE-Dubai-80-MS 1368.9/09
Datasets
UVRR: UV-Resonance Raman with 244 nm excitation on bulk samples, calibration standard Polystyrene, measurements were time series of 10 consecutive spectra, for each strain and batch 25 time series were collected from 3 different slides
532nm: Single cell analysis with 532nm excitation, calibration standard 4AAP, one spectrum per bacterial cell was collected
785nm: Bulk analysis of bacterial colonies using 785 nm excitation and a Raman fibre probe, calibration standard 4AAP, bulk analysis, individual spectra of colonies were collected
Data structure is in the metadata files.
Individual spectra are in the folders sorted by the date they were measured.