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RT-qPCR dCT values of genes analyzed in colon, small intestine, liver and colon organoids

Núñez-Sánchez, María A.; Herisson, Florence M.; García-González, Natalia; Rossini, Valerio; Pinhiero, Jorge; Daly, Jack; Bustamante-Garrido, Milán; Hueston, Cara M.; Patel, Shriram; Canela, Nuria; Herrero, Pol; Claesson, Marcus; Melgar, Silvia; Nally, Ken; Caplice, Noel M.; Gahan, Cormac G.M.

RT-qPCR dCt values of gene expression from colon, small intestine and liver tissues from germ free mice monocolonized with E. coli expression bile salt hydrolase activity. Mice monocolonized with non-BSH expressing E. coli, non-monocolonized and conventionally-raised mice were used as controls, and RT-qpCR dCT values of colon organoids incubated with a mix of bile acids, LPS or a mix of bile acids + LPS. Values are normalized to the expression levels of ß-actin

 

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