Published May 31, 2022
| Version v1
Dataset
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RT-qPCR dCT values of genes analyzed in colon, small intestine, liver and colon organoids
Creators
- 1. Obesity and Metabolism Laboratory, Biomedical Research Insitituto of Murcia, Murcia, Spain
- 2. Centre for Research in Vascular Biology, University College Cork, Cork, Ireland.
- 3. APC Microbiome Ireland, University College Cork, Cork, Ireland.
- 4. Eurecat,Centre Tecnològic de Catalunya, Centre for Omic Sciences (COS), Joint Unit Universitat Rovira i Virgili-EURECAT, Reus, Spain
Description
RT-qPCR dCt values of gene expression from colon, small intestine and liver tissues from germ free mice monocolonized with E. coli expression bile salt hydrolase activity. Mice monocolonized with non-BSH expressing E. coli, non-monocolonized and conventionally-raised mice were used as controls, and RT-qpCR dCT values of colon organoids incubated with a mix of bile acids, LPS or a mix of bile acids + LPS. Values are normalized to the expression levels of ß-actin
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