Published June 7, 2021 | Version v1
Journal article Open

The emerging landscape of single-molecule protein sequencing technologies

Creators

  • 1. International Centre for Cancer Vaccine Science, University of Gdańsk, Gdańsk, Poland
  • 2. Faculty of Applied Sciences, Delft University of Technology, Delft, the Netherlands
  • 3. Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA
  • 4. Department of BioNanoScience, Kavli Institute of Nanoscience, Delft University of Technology, Delft, the Netherlands
  • 5. Department of Chemistry, University of Texas at Austin, Austin, TX, USA
  • 6. Department of Biomedical Engineering, Technion–Israel Institute of Technology, Haifa, Israel
  • 7. NanoDynamicsLab, Laboratory of Biophysics, Wageningen University, Wageningen, the Netherlands
  • 8. Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL, USA
  • 9. Institute of Bioengineering, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
  • 10. Dipartimento di Ingegneria Industriale, Università di Roma Tor Vergata, Rome, Italy
  • 11. Univ. Lille, Inserm, CHU Lille, U1192– Protéomique Réponse Inflammatoire Spectrométrie de Masse–PRISM, Lille, France
  • 12. Department of Infection and Immunity, Luxembourg Institute of Health, Strassen, Luxembourg
  • 13. Department of Physics, Brown University, Providence, RI, USA
  • 14. Université Grenoble Alpes, CEA, LETI, Grenoble, France
  • 15. Departments of Chemistry and Molecular Biosciences, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA
  • 16. Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA
  • 17. Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology, University of Maryland, Rockville, MD, USA
  • 18. Chair of Proteomics and Bioanalytics, Technische Universität München, Freising, Germany
  • 19. Department of Biochemistry and Molecular Biology, Biofisika Institute (CSIC, UPV/EHU), Leioa, Spain
  • 20. Biodesign Institute, School of Molecular Sciences, Department of Physics, Arizona State University, Tempe, AZ, USA
  • 21. Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, the Netherlands
  • 22. Department of Molecular Biosciences, Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, TX, USA
  • 23. Université Grenoble Alpes, CEA, Inserm, BGE U1038, Grenoble, France
  • 24. Adolphe Merkle Institute, University of Fribourg, Fribourg, Switzerland
  • 25. University of Toronto, Hospital for Sick Children, Toronto, Ontario, Canada
  • 26. Department of Physics, Northeastern University, Boston, MA, USA

Description

Single-cell profiling methods have had a profound impact on the understanding of cellular heterogeneity. While genomes and transcriptomes can be explored at the single-cell level, single-cell profiling of proteomes is not yet established. Here we describe new single-molecule protein sequencing and identification technologies alongside innovations in mass spectrometry that will eventually enable broad sequence coverage in single-cell profiling. These technologies will in turn facilitate biological discovery and open new avenues for ultrasensitive disease diagnostics.

Files

s41592-021-01143-1-2.pdf

Files (2.0 MB)

Name Size Download all
md5:7f11416b97e9bd7e92a66e1d9199f35a
2.0 MB Preview Download

Additional details

Funding

European Commission
MONET - Merkel cell polyomavirus Oncogenic Network 843052