Published May 11, 2022 | Version v1
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Characterization of hypoxia-inducible factor α and its response to hypoxia in Callosobruchus chinensis (Coleoptera, Bruchidae)

  • 1. Jiangsu University of Science and Technology
  • 2. University of Groningen
  • 3. Shanghai Jiao Tong University

Description

Hypoxia-inducible factor (HIF) plays an essential role in hypoxic adaptation in various organisms. Callosobruchus chinensis, a common food storage pest, is highly adapted to hypoxic conditions, while the underlying mechanism of which is currently unclear. Here, we isolated, sequenced, and bioinformatically analyzed HIF1α from C. chinensis, then examined its mRNA and protein expression profiles under hypoxic conditions for various developmental stages and tissue types. The effects of environmental hypoxia on other genes in the HIF pathway response, aspartyl-hydroxylase (FIH) and prolyl hydroxylase (PHD), were also examined. We found that FIH and PHD were also highly conserved and that their expression varied with developmental stage, tissue type, and environmental hypoxia. Overall, hypoxia caused an increase in HIF1α and FIH mRNA expression, and a decrease in PHD mRNA expression, while expression of their proteins showed different in response to hypoxia. HIF1α protein levels increased, PHD and FIH protein levels decreased under hypoxic conditions, compared to normoxic conditions. This study has preliminarily investigated the bioinformation of C. chinensis HIF1α response relationship between HIF1α and its hydroxylases under normoxic and hypoxic conditions, which might provide important clues for future work on clarifying the mechanism of stored-product insect unique hypoxia adaption.

Notes

See README file.

Funding provided by: Jiangsu University of Science and Technology
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100012216
Award Number: 1182931803

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