Published September 4, 2019
| Version V3.0
Technical note
Open
Quality Control Characterization of α-Synuclein Preformed Fibrils (PFFs)
Creators
- 1. MNI, McGill University
Description
This protocol describes how to:
- Determine fibril length of sonicated unlabelled α-synuclein preformed fibrils (PFFs) by dynamic light scattering (DLS) and electron microscopy (EM)
- Confirm cellular uptake of fluorescently-labelled α-synuclein PFFs by immunofluorescence (IF)
- Qualitatively verify the formation of amyloid beta-sheets in α -synuclein PFF by Thioflavin (ThT) assay.
Version 3.0 now available.
Files
EDDU-007-03_Quality Control Characterization of α-Synuclein Preformed Fibrils (PFFs)_v3.0.pdf
Files
(3.3 MB)
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