SGC Open Notebooks
Published September 4, 2019 | Version V3.0
Technical note Open

Quality Control Characterization of α-Synuclein Preformed Fibrils (PFFs)

Creators

  • 1. MNI, McGill University

Description

This protocol describes how to:

  • Determine fibril length of sonicated unlabelled α-synuclein preformed fibrils (PFFs) by dynamic light scattering (DLS) and electron microscopy (EM)
  • Confirm cellular uptake of fluorescently-labelled α-synuclein PFFs by immunofluorescence (IF)
  • Qualitatively verify the formation of amyloid beta-sheets in α -synuclein PFF by Thioflavin (ThT) assay.

Version 3.0 now available. 

Files

EDDU-007-03_Quality Control Characterization of α-Synuclein Preformed Fibrils (PFFs)_v3.0.pdf

Files (3.3 MB)