Technical note Open Access
Quality Control Characterization of α-Synuclein Preformed Fibrils (PFFs)
Del Cid Pellitero, Esther; Shlaifer, RIna; Luo, Wen; Krahn, Andrea; Nguyen-Renou, Emmanuelle; Manecka, Destiny-Love; Rao, Trisha; Beitel, Lenore; Durcan, Thomas M.; Pimentel, Luisa
This protocol describes how to:
- Determine fibril length of sonicated unlabelled α-synuclein preformed fibrils (PFFs) by dynamic light scattering (DLS) and electron microscopy (EM)
- Confirm cellular uptake of fluorescently-labelled α-synuclein PFFs by immunofluorescence (IF)
- Qualitatively verify the formation of amyloid beta-sheets in α -synuclein PFF by Thioflavin (ThT) assay.
Version 3.0 now available.
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EDDU-007-03_Quality Control Characterization of α-Synuclein Preformed Fibrils (PFFs)_v3.0.pdf
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Indexed in
- Publication date:
- September 4, 2019
- DOI:
-
- Keyword(s):
- alpha-synuclein quality-control fluorescent imaging electron microscopy dynamic light scattering (DLS)
- Communities:
- License (for files):
- Creative Commons Attribution 4.0 International