Technical note Open Access

Quality Control Characterization of α-Synuclein Preformed Fibrils (PFFs)

Del Cid Pellitero, Esther; Shlaifer, RIna; Luo, Wen; Krahn, Andrea; Nguyen-Renou, Emmanuelle; Manecka, Destiny-Love; Rao, Trisha; Beitel, Lenore; Durcan, Thomas M.; Pimentel, Luisa

This protocol describes how to:

  • Determine fibril length of sonicated unlabelled α-synuclein preformed fibrils (PFFs) by dynamic light scattering (DLS) and electron microscopy (EM)
  • Confirm cellular uptake of fluorescently-labelled α-synuclein PFFs by immunofluorescence (IF)
  • Qualitatively verify the formation of amyloid beta-sheets in α -synuclein PFF by Thioflavin (ThT) assay.

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