Pan-cancer analysis for immune cell infiltration and mutational signatures using non-negative canonical correlation analysis

Mutational signatures indicate the mutational processes and substitution patterns in cancer 13
cell genomes. However, the functional consequences of mutational signatures remain unclear. Here, 14
the research investigated the relationship between mutational signatures and immune cell infiltration 15
using non-negative canonical correlation analysis, based on more than 8,900 patients across 25 16
tumor types. By inspecting mutational signatures with the maximal coefficients searched by the 17
non-negative canonical correlation analysis, the study identified the mutational signatures related 18
to the immune cell infiltration composed of the tumor microenvironments. The analysis validated 19
by showing that the genes associated the identified mutational signatures were linked to overall 20
survival by a Kaplan-Meier curve and a log-rank test and were mainly related to immunity by gene 21
set enrichment analysis. These results will help expand our knowledge of tumor biology and to 22
recognize the functional roles and association of immune systems with mutational signatures.