Paramecium Polycomb Repressive Complex 2 physically interacts with the small RNA binding PIWI protein to repress transposable elements
Creators
- 1. Université de Paris, CNRS, Institut Jacques Monod, F-75006, Paris, France
- 2. Institut Curie, Paris Sciences et Lettres Research University, INSERM, CNRS, Paris 75005, France.
- 3. Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France
- 4. Université de Paris, CNRS, Laboratoire Epigénétique et Destin Cellulaire, F-75013, Paris, France.
Description
Polycomb Repressive Complex 2 (PRC2) maintains transcriptionally silent genes in a repressed state via deposition of histone H3 K27 trimethyl (me3) marks. PRC2 has also been implicated in silencing transposable elements (TEs), yet how PRC2 is targeted to TEs remains unclear. To address this question, we identified proteins that physically interact with the Paramecium Enhancer-of-zeste Ezl1 enzyme, which catalyzes H3K9me3 and H3K27me3 deposition at TEs. We show that the Paramecium PRC2 core complex comprises four subunits, each required in vivo for catalytic activity. We also identify PRC2 cofactors, including the RNA interference (RNAi) effector Ptiwi09, which are necessary to target H3K9me3 and H3K27me3 to TEs. We find that the physical interaction between PRC2 and the RNAi pathway is mediated by a RING finger protein and that small RNA recruitment of PRC2 to TEs is analogous to the small RNA recruitment of H3K9 methylation SU(VAR)3-9 enzymes.
Files
Miro_Pina_et_al_Zenodo_v3.zip
Files
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Additional details
Related works
- Is cited by
- 10.1101/2021.08.12.456067 (DOI)
Funding
- LaMarque – Evolutionary transitions in genomic discrimination of self and non-self: small RNAs, sequence-specific factors and DNA methylation ANR-18-CE12-0005
- Agence Nationale de la Recherche
- POLYCHROME – Polycomb Repressive Complex in Paramecium: interaction with small RNA machinery and substrate specificity ANR-19-CE12-0015
- Agence Nationale de la Recherche
- WHO AM I – Determinants de l'Identité : de la molécule à l'individu 11-LABX-0071
- Agence Nationale de la Recherche