iPSpine H2020
Published December 31, 2020 | Version v1
Journal article Open

DIPPER, a spatiotemporal proteomics atlas of human intervertebral discs for exploring ageing and degeneration dynamics

Creators

  • 1. School of Biomedical Sciences, , The University of Hong Kong, Hong Kong
  • 2. Centre for Blood Research, Faculty of Dentistry, University of British Columbia, Vancouver, Canada
  • 3. Proteomics and Metabolomics Core Facility, The University of Hong Kong, Hong Kong
  • 4. Department of Surgery, McGill University, Montreal, Canada
  • 5. Centre for PanorOmic Sciences (CPOS), The University of Hong Kong, Hong Kong

Description

The spatiotemporal proteome of the intervertebral disc (IVD) underpins its integrity and function. We present DIPPER, a deep and comprehensive IVD proteomic resource comprising 94 genome-wide profiles from 17 individuals. To begin with, protein modules defining key directional trends spanning the lateral and anteroposterior axes were derived from high-resolution spatial proteomes of intact young cadaveric lumbar IVDs. They revealed novel region-specific profiles of regulatory activities and displayed potential paths of deconstruction in the level- and location-matched aged cadaveric discs. Machine learning methods predicted a ‘hydration matrisome’ that connects extracellular matrix with MRI intensity. Importantly, the static proteome used as point-references can be integrated with dynamic proteome (SILAC/degradome) and transcriptome data from multiple clinical samples, enhancing robustness and clinical relevance. The data, findings, and methodology, available on a web interface (http://www.sbms.hku.hk/dclab/ DIPPER/), will be valuable references in the field of IVD biology and proteomic analytics.

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DIPPER_a_spatiotemporal_proteomics_atlas_of_human_intervertebral_discs_for_exploring_ageing_and_degeneration_dynamics.pdf

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Additional details

Funding

iPSpine – Induced pluripotent stem cell-based therapy for spinal regeneration 825925
European Commission