Spermogram and Hormone Profile Indicators Analysis Among Patients with Different Types of Male Infertility Depending on Antioxidant System Status
- 1. Odessa National Medical University, Center of Reconstructive and Restorative Medicine at the ONMU, Ukraine
- 2. Odessa National Medical University, Ukraine
- 3. State Enterprise Ukrainian Research Institute of Transport Medicine of the Ministry of Health of Ukraine, Ukraine
Description
The objective: evaluation of interrelation between indicators of spermoplasm, androgen profile and malondialdehyde (MDA) content in spermoplasm among men with different types of male infertility.
Materials and methods. 39 men at the age ranged from 24 to 45 years with different degrees of spermatogenesis and hormonal profile disorders were examined; MDA content in spermoplasm among patients who were diagnosed with «secretory male infertility» (n=7) was 23.3-38.3 μm/l, diagnosed with «excretory-toxic male infertility» (n=32) – 17.3–53.0 μm/l, therefore this group was divided into subgroups: 1 – up to 19.9 μm/l; 2 – within 20.6–29.1 mcm/l; 3 – more than 30.0 μm/l. In spermoplasm of the control group (donor sperm), this indicator was within 20.8–24.9 μm/l range. Interrelation assessment between the investigated indicators was carried out by methods of variational and correlation analysis using non-parametric statistics.
Results. Studies results showed that the number of white blood cells in patients’ sperm of all groups exceeded the standard, wherein a close connection between this indicator and MDA content in spermoplasm (r=0.99 and r=0.48, with α≤0,05) was established. In groups where lipid peroxidation activity was significantly higher than control indicators, the highest percentage of men (more than 40%) with reduced sperm activity and viability was detected.
Free testosterone decrease was observed among almost 70-90% of examined men, while the most significant changes were found among patients who were diagnosed with «excretory-toxic male infertility» and high MDA content in spermoplasm. A close positive connection between MDA content and sperm dilution time (r=0.79; α≤0,05) was established in group of patients who were diagnosed with «secretory male infertility», as well as the level of LH in blood (r=0.74; α≤0,05); among patients who were diagnosed with «excretory-toxic male infertility» the status of the pro-antioxidant system, which is characterized as «oxidative stress» (third subgroup), a reliable negative association was found between MDA level in spermoplasm and the level of FSH in blood (r=– 0.75; α≤0,05).
Conclusions. A relation between high MDA level in spermoplasm and increase of white blood cells number in sperm was established, as well as a decrease of sperm motor activity and content of bioavailable testosterone in blood.
Files
Spermogram_and_Hormone_Profile_Indicators_Analysis_Among_Patients_with_Different_Types_of_Male_Infertility_Depending_on_Antioxidant_System_Status.pdf
Files
(1.3 MB)
| Name | Size | Download all |
|---|---|---|
|
md5:eb9fa2a087984b40dc8f60ac3e2bd9bd
|
1.3 MB | Preview Download |
Additional details
References
- Amorini A, Listorti I, Bilotta G, Pallisco R, Saab M, Mangione R. et al. Antioxidant-Based Therapies in Male Infertility: Do We Have Sufficient Evidence Supporting Their Effectiveness? Antioxidants (Basel). 2021;10(2):220. doi: 10.3390/antiox10020220.
- Antomonov M.U. Mathematical processing and analysis of biomedical data. Kyiv: Medinform; 2018. 579 р.
- Fatima S, Alwaznah R, Aljuraiban G, Wasi S, Abudawood M, Abulmeaty M, et al. Effect of seminal redox status on lipid peroxidation, apoptosis and DNA fragmentation in spermatozoa of infertile. Saudi Med J. 2020;41(3):238-46. doi: 10.15537/smj.2020.3.24975.
- Gorpynchenko ІІ, Gurzhenko YuM, Spyrydonenko VV, Litvinets EA. Influence of radioactive background on an idiopathic form of infertility incidence in men from different regions of Ukraine. Health man. 2021;1:47-52. doi: 10.30841/2307-5090.1.2021.232477.
- Khmil SV, Khmil МS. Achievements and prospects of development in modern methods of supporting reproductive technologies in infertility treatment. Adv Clin Experimental Med. 2015;4:3-7.
- Mahdian S, Shahhoseini M, Moini A. COVID-19 Mediated by Basigin Can Affect Male and Female Fertility. Int J Fertil Steril. 2020;14(3):262-3. doi: 10.22074/ijfs.2020.134702.
- Nowicka-Bauer K, Nixon B. Molecular Changes Induced by Oxidative Stress that Impair Human Sperm Motility. Antioxidants (Basel). 2020;9(2):134. doi: 10.3390/antiox9020134.
- Povorozniuk МV. State of reproductive health among men in marriage. Med Aspects Man's Health. 2019;4(35):51-7.
- President of Ukraine Decree. About additional measures concerning state support of family, protection of motherhood, fatherhood and childhood [Internet]. 214/2019. Available from: 2019 May 11.
- Suprunenko SМ. Main aspects of metabolic syndrome and male infertility dependence (literature review). Bulletin of biology and medicine problems. 2020;3(157):35-40.