Dataset Restricted Access
#GliaMorph - Double-transgenic Müller Glia Data at 3dpf and 5dpf Zebrafish
Elisabeth Kugler;
Alicia Carrington;
Ryan B. MacDonald
Images of zebrafish retina at 3dpf and 5dpf in the double-transgenic Tg(TP1bglob:VenusPest)s940 (Ninov et al., 2012) and Tg(CSL:mCherry)jh11 (also known as Tg(Tp1bglob:hmgb1-mCherry)jh11) (Parsons et al., 2009).
GliaMorph toolkit to quantify glia shape in 3D: https://github.com/ElisabethKugler/GliaMorph
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step1_cziToTiffTool.ijm: Macro for czi to tiff conversion
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step2_DeconvolutionTool.ijm: Macro for deconvolution of confocal images using theoretical or experimental PSF
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step3_90DegreeRotationTool.ijm: Macro for 90 degree rotation - optional before rotationTool
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step4_subregionTool.ijm: Semi-automatic ROI selection tool with ROI selection from MIP
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step4_subregionToolWithinStack.ijm: Semi-automatic ROI selection tool within stack with ROI within stack
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step5_splitChannelsTool.ijm: Macro to split channels and save them in separate folders
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step6_zonationTool.ijm: Analysis of zebrafish retina zonation based on intensity profiles
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step7_SegmentationTool.ijm: Segmentation of MG cells
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step8_QuantificationTool.ijm: Quantification of MG features in segmented images
Restricted Access
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Data made available upon reasonable request.
| All versions | This version | |
|---|---|---|
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| Data volume | 44.6 GB | 44.6 GB |
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| Unique downloads | 5 | 5 |
- Publication date:
- February 1, 2022
- DOI:
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- Keyword(s):
- zebrafish, retina, glia, neuroscience, opthalmology, quantification