Published June 15, 2014 | Version v1
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PROTECTIVE EFFECT OF 1-DEOXYNOJIRIMYCIN AND RESVERATROL AGAINST DIABETIC OXIDATIVE STRESS AND INFLAMMATION

Creators

  • 1. Faculty of Geography and Biology, Pedagogical University, Kraków, Poland,
  • 2. Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Slovakia

Description

Diabetes mellitus (DM) was induced in groups II, V, and VI. Animals III experimental group was administered 1-deoxynojirimycin (DNJ) in dose 0,75 mgKg-1 body weight/day for 14 days. Mice IV experimental group was administered resveratrol (RSV) in dose 30 mgKg-1 body weight/day for 14 days. Animals group V: diabetic group receiving DNJ, group VI: diabetic group receiving RSV. Thirty minutes after the last injection animals were anaesthetized and decapitated. Serum level of interleukin-6 (IL-6) was assessed as surrogate markers of inflammation. The livers levels of reduced glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT) and superoxide dismutase (SOD) activity were measured as surrogate markers of oxidative stress. Serum level of insulin and plasma glucose levels were assessed as surrogate markers of diabetes. The administration of DNJ or RSV significantly restored glucose, insulin, IL-6, GSH levels and SOD, CAT, GPx activity in the diabetic mice to near control. The results demonstrated that DNJ and RSV with its antidiabetic, antiinflammation and antioxidant properties could be a potential herbal medicine in treating diabetes and hepatic problems.

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6. GREŃ et al. 2014 Vol. 63, No. 1-2 pp. 44-54.pdf

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