Published June 28, 2021
| Version 1.0
Journal article
Open
SangeR: The high-throughput Sanger sequenc-ing analysis pipeline
Creators
- 1. Department of Neuropathology Justus-Liebig University Giessen, Arndtstr. 16 D-35392 Giessen
- 2. Department of Medical Informatics Justus-Liebig University Giessen, Rudolf-Buchheim-Str. 6 D-35396 Giessen
Description
Summary:
In the era of Next Generation Sequencing and beyond, the Sanger technique is still widely used for variant verification tasks of ambiguous high-throughput sequencing results or as cheap molecular genetical analysis of singular targets in many fields of study. Nevertheless, many analysis steps need time consuming manual intervention. Therefore, we here present a pipeline-able high-throughput solution with optional R-Shiny web-interface, delivering a binary mutation decision of hotspots together with plotted chromatograms including annotations via flat files.
Files
ab1_with_mutation.zip
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