ANTI-CANCER POTENTIAL IN METHANOL EXTRACT OF BLACK BAKAU LEAVES (Rhizophora mucronata Poiret) USING THE Brine Shrimp Lethality Test (BSLT) METHOD

Cancer is a disease of major problem in the world. In 2018 an estimated death rate of 9.6 million due to cancer. The prevalence of cancer in Indonesia is 1.4% or an estimated 347.792 people in 2013. Cancer is a disease caused by the growth of body tissue cells that are not normal. Chemotherapy is a cancer treatment, but the drugs used in chemotherapy are very powerful, so it can kill cancer cells and attack healthy cells. Based on these side effects, in this study research related to new alternatives as a cancer treatment. Rhizophora mucronata is a mangrove that has secondary metabolites such as flavonoids, terpenoids, and saponins that have a potential as anticancer agents. In this study to analyze the potential of anticancer activity and secondary metabolites contained of mangrove leaves extract of Rhizophora mucronata Poiret were obtained from Pamurbaya, Surabaya, East Java. In this study, the Brine Shrimp Lethality Test was used as an anticancer prescreening test and LC50 (Letal concentration) value was used as a parameter. Methanol extract of Rhizophora mucronata leaves with a concentration of 10.1; 20.2; 60.6; 101; 202; 505; 1010 ppm was given to Artemia salina as a test solution. And the result of this study is the methanol extract of Rhizophora mucronata Poiret leaves which have potential as anticancer with LC50 values of 170.6082 ppm with the results of phytochemical screening containing secondary metabolites such as tannins, saponins, flavonoids, alkaloids, terpenoids, polyphenols, anthraquinones, and steroids.