In Vivo Transcriptomic Profiling using Cell Encapsulation Identifies Effector Pathways of Systemic Aging
Creators
- Mashinchian, Omid1
- Hong, Xiaotong2
- Michaud, Joris2
- Migliavacca, Eugenia3
- Lefebvre, Gregory3
- Boss, Christophe3
- De Franceschi, Filippo3
- Le Moal, Emmeran4
- Collerette-Tremblay, Jasmin4
- Isern, Joan5
- Metairon, Sylviane3
- Raymond, Frederic3
- Descombes, Patrick3
- Bouche, Nicolas3
- Muñoz-Cánoves, Pura6
- Feige, Jerome N.1
- Bentzinger, C. Florian7
- 1. Nestlé Institute of Health Science, Nestlé Research, Société des Produits Nestlé S.A., Lausanne, Switzerland - School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
- 2. Nestlé Institute of Health Science, Nestlé Research, Société des Produits Nestlé S.A., Lausanne, Switzerland - Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain
- 3. Nestlé Institute of Health Science, Nestlé Research, Société des Produits Nestlé S.A., Lausanne, Switzerland
- 4. Département de Pharmacologie-Physiologie, Institut de Pharmacologie de Sherbrooke, Centre de Recherche du Centre Hospitalier Universitaire de Sherbrooke, Faculté de Médecine et des Sciences de la Santé, Université de Sherbrooke, Sherbrooke, Canada
- 5. Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain
- 6. Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain - Department of Experimental and Health Sciences, Pompeu Fabra University (UPF), CIBER on Neurodegenerative Diseases (CIBERNED) and Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain
- 7. Nestlé Institute of Health Science, Nestlé Research, Société des Produits Nestlé S.A., Lausanne, Switzerland - Département de Pharmacologie-Physiologie, Institut de Pharmacologie de Sherbrooke, Centre de Recherche du Centre Hospitalier Universitaire de Sherbrooke, Faculté de Médecine et des Sciences de la Santé, Université de Sherbrooke, Sherbrooke, Canada
Description
Abstract
Sustained exposure to a young systemic environment rejuvenates aged organisms and promotes cellular function. However, due to the intrinsic complexity of tissues it remains challenging to pinpoint niche-independent effects of circulating factors on specific cell populations. Here we describe a method for the encapsulation of human and mouse skeletal muscle progenitors in diffusible polyethersulfone hollow fiber capsules that can be used to profile systemic aging in vivo independent of heterogeneous short-range tissue interactions. We observed that circulating long-range signaling factors in the old systemic environment lead to an activation of Myc and E2F transcription factors, induce senescence and suppress myogenic differentiation. Importantly, in vitro profiling using young and old serum in 2D culture does not capture all pathways deregulated in encapsulated cells in aged mice. Thus, in vivo transcriptomic profiling using cell encapsulation allows for the characterization of effector pathways of systemic aging with unparalleled accuracy.
Files
Mashinchian et al 2021.pdf
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