ISOLATION AND CHARACTERIZATION OF QUERCETIN IN MATHUKA NERIIFOLIA (MOON) H.J. LAM. ETHANOL EXTRACT

Herbal medicine is making a dramatic comeback as the side effect of synthetic medicine are daunting and the therapeutic approach is drifting towards substituent medicine. Quercetin is a plant pigment flavonoid. It's found in many plants and foods, such as red wine, onions, green tea, apples, and berries. Quercetin has antioxidant and anti-inflammatory effects that might help reduce swelling, kill cancer cells, control blood sugar, and help prevent heart disease. Madhuca neriifolia (Moon) H.J. Lam. is an endangered plant species occurring in the Southern, Western Ghats of India. Flowers are used in the treatment of kidney complaints. Fruits are recommended in cases of rheumatism, biliousness, consumption, asthma and worm trouble. Oil from seeds is used to treat rheumatism and for improved growth of hair. Fractnation of ethanol extract  of Mathuka Neriifolia (Moon) H.J. Lam. by column chromatography was done. During the column elution process, the fractions 260 - 300  has a single banding pattern which was confirmed by TLC study. Phyochemical screening showed the presence of Flavonoids , Phenolic and Tannins. Physical and chemical tests  of isolated compound was conducted. By using IR, LC-MS, ¹H-NMR and ¹³C-NMR spectra of the isolated compound was confirmed as Quercetin.

Keywords: Quercetin, Mathuka Neriifolia (Moon) H.J. Lam., Phytochemical screening, flavonoid.