Published December 15, 2015 | Version v1
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EFFECT OF GONADOTROPHIN (PERGONAL®) ON HAEMATOLOGY, IMMUNE/LEUCOCYTE STATUS AND SERUM METABOLITES OF MATURE WEST AFRICAN DWARF RAMS TREATED FOR SPERM PRODUCTION

Creators

  • 1. Department of Animal Science and Fisheries, Abia State University, Umuahia Campus. AbiaState, Nigeria

Description

Twenty–four (24)West African Dwarf (WAD) rams aged 2 – 3 years were used to determine the effect of gonadotrophin (Pergonal®) on haematology, immune /leucocyte status and serum metabolites of mature rams treated for sperm production. The Completely Randomized Design (CRD) was used to assign the rams to 4 treatment groups consisting of 6 rams per treatment with one ram per replicate. These groups were assigned to 4 levels of Pergonal® injection as treatments. The doses were 0.00 u/L, 19.26 u/L, 39.00u/L and 57.78 u/L Pergonal® represented as T1 T2, T3 and T4 respectively. The group which received 0.00 I.U Pergonal® served as the control. All the treatments were administered by intramuscular injections. The results showed significant differences (P < 0.05) among the treatment groups in Packed Cell Volume (PCV), Haemoglobin (Hb), White Blood Cell (WBC) and Mean Corpuscular Volume (MCV) values. However, there were no significant differences (P > 0.05) among the treatment groups in red blood cells (RBC), Mean Corpuscular Haemoglobin (MCH), and Mean Corpuscular Haemoglobin Concentration (MCHC) values. The highest values for PCV (48%), Hb (16.25g/dl),WBC (11.5 x109/L),RBC (5.35 x1012/L) and MCV(90.50fl)were obtained from rams on 39.00 I.U Pergonal(T3).There were also no significant differences (P>0.05) among the treatment groups in neutrophils, eosinophils, and monocytes values. However, there were significant differences (P<0.05) among the treatment group in lymphocyte values which increased as the administered dosage of Pergona®l increased. The result further showed that there were significant differences (P<0.05) among the treatment groups in serum cholesterol, glucose, calcium, total bilirubin and conjugated bilirubin values. Cholesterol values increased significantly as the dosage of Pergona®l increased. The results of this study showed that apart from RBC, MCH, MCHC, neutrophils, and basophils, the haematological and serum biochemical parameters, and immune status of West African dwarf rams may be affected when 19.26 u/L or more of Pergonal® are used for induction of spermatogenesis, and so they should be constantly monitored.

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